PMID: 7546289Oct 1, 1995Paper

Use of the teleost saccule to identify genes involved in inner ear function

DNA and Cell Biology
J G DavisM I Greene

Abstract

The vertebrate inner ear sensory epithelia contain different types of hair cells and supporting cells. The teleost saccule is anatomically similar to the mammalian saccule and is primarily involved in the detection of translational acceleration and orientation with respect to gravity. To facilitate molecular studies of the teleost saccule cDNA libraries were constructed from microdissected Lepomis macrochirus (bluegill sunfish) saccular maculae. To our knowledge, this is the first report of cDNA libraries constructed from the saccule. In one instance, a non-polymerase chain reaction-based method of amplifying a mRNA population from limited amounts of starting tissue was employed that allowed construction of cDNA libraries from nanogram amounts of tissue mRNA. Conventional cDNA libraries were constructed from the sunfish saccular maculae as well. These cDNA libraries enriched in hair cell and supporting cell transcripts should facilitate molecular biological studies of inner ear sensory epithelia. As an example of their utility, efforts to identify tyrosine kinases expressed in the saccular endorgan using low-stringency hybridization screening of these cDNA libraries and the partial sequence of a cDNA found to encode an erbB-2-r...Continue Reading

References

Dec 1, 1992·Hearing Research·R KillickG P Richardson
Jun 1, 1992·Hearing Research·J C OberholtzerF M Matschinsky
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R N Van GelderJ H Eberwine
Jan 1, 1990·Hearing Research·K M Khan, D G Drescher
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·K G Pote, M D Ross
Jan 1, 1991·Annual Review of Neuroscience·J T Corwin, M E Warchol
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Apr 25, 1989·Nucleic Acids Research·A BelyavskyK Rajewsky
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G M ShepherdD P Corey
Sep 11, 1987·Cell·T Hunter
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J C OberholtzerF M Matschinsky
Jan 1, 1987·Hearing Research·G P RichardsonA J Bailey
Jan 1, 1980·Acta Oto-laryngologica·K Steel
Oct 1, 1981·The Journal of Comparative Neurology·J T Corwin
Aug 1, 1994·Hearing Research·H S LiB J Morley
Apr 1, 1995·The Journal of Cell Biology·R KillickG P Richardson
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·E E KarrerD Alexander
Nov 1, 1994·Trends in Biochemical Sciences·E G Krebs
Feb 1, 1994·Progress in Neurobiology·S KorneevJ A Davies
Nov 1, 1993·Archives of Otolaryngology--head & Neck Surgery·A F RyanJ P Harris
Jan 1, 1993·Brain, Behavior and Evolution·A N Popper, R R Fay
Feb 1, 1993·Current Opinion in Neurobiology·J T CorwinM W Kelley
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·P G Gillespie, A J Hudspeth

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Citations

Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·J G DavisM I Greene
Feb 16, 2007·Hearing Research·Michael S HildebrandHans-Henrik M Dahl

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