Use of the transposon Ac as a gene-searching engine in the maize genome

The Plant Cell
Matthew CowperthwaiteHugo K Dooner

Abstract

We show here that, although genes constitute only a small percentage of the maize genome, it is possible to identify them phenotypically as Ac receptor sites. Simple and efficient Ac transposition assays based on the well-studied endosperm markers bz and wx were used to generate a collection of >1300 independent Ac transposants. The majority of transposed Ac elements are linked to either the bz or the wx donor loci on chromosome 9. A few of the insertions produce obvious visible phenotypes, but most of them do not, suggesting that these populations will be more useful for reverse genetics than for forward transposon mutagenesis. An inverse polymerase chain reaction method was adapted for the isolation of DNA adjacent to the transposed Ac elements (tac sites). Most Ac insertions were into unique DNA. By sequencing tac sites and comparing the sequences to existing databases, insertions were identified in a number of putative maize genes. The expression of most of these genes was confirmed by RNA gel blot analysis. We report here the isolation and characterization of the first 46 tac sites from the two insertion libraries.

References

Jun 1, 1989·Molecular & General Genetics : MGG·P S SchnableH Saedler
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·V L Chandler, V Walbot
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K C ConeB Burr
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·N CarelsG Bernardi
Jan 1, 1995·The Plant Cell·R J BensenS P Briggs
Mar 1, 1995·The Plant Cell·L Das, R Martienssen
Aug 1, 1994·Genome Génome / Conseil National De Recherches Canada·J L BennetzenP SanMiguel
Dec 1, 1995·The Plant Journal : for Cell and Molecular Biology·E TackeH P Döring
Sep 1, 1996·The Plant Journal : for Cell and Molecular Biology·R TsugekiN V Fedoroff
Nov 1, 1996·Science·P SanMiguelJ L Bennetzen
Feb 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·J W ThatcherW J Dickinson
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·E WismanB Weisshaar
Oct 3, 1999·The Plant Journal : for Cell and Molecular Biology·H EnokiK Shimamoto
Oct 3, 1999·The Plant Journal : for Cell and Molecular Biology·H G ChinC D Han
Feb 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·Q ZhangS R Wessler
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·Q H LeT Bureau
Dec 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·B ShenH K Dooner
Dec 29, 2000·Nature·UNKNOWN Arabidopsis Genome Initiative
Feb 13, 2001·The Plant Journal : for Cell and Molecular Biology·K TurcotteT Bureau
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·H FuH K Dooner

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Citations

Apr 20, 2010·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Federico MartinA Mark Settles
Jun 29, 2010·Functional & Integrative Genomics·Lam-Son Phan Tran, Keiichi Mochida
Nov 23, 2011·Functional & Integrative Genomics·Surinder SinghJaswinder Singh
Oct 12, 2005·Plant Molecular Biology·Gynheung AnSichul Lee
Jul 6, 2007·Plant Molecular Biology·Sung Han ParkChang-Deok Han
Feb 13, 2001·Current Opinion in Chemical Biology·L HamerJ E Hamer
Apr 2, 2003·Current Opinion in Plant Biology·Pablo D RabinowiczRobert A Martienssen
Jan 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Huihua FuHugo K Dooner
May 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Limei He, Hugo K Dooner
Jun 24, 2009·Plant & Cell Physiology·Lakshmi P ManavalanHenry T Nguyen
Jan 8, 2009·Plant Physiology·Joachim Messing
Nov 6, 2012·Plant Physiology·Yaya CuiGary Stacey
Apr 3, 2003·The Plant Cell·Manjit SinghThomas P Brutnell
Jun 29, 2010·The Plant Cell·Erik VollbrechtThomas P Brutnell
Jun 2, 2009·Genome Génome / Conseil National De Recherches Canada·Harpinder S RandhawaKulvinder S Gill
Jan 19, 2012·International Journal of Plant Genomics·Ethalinda K S CannonCarolyn J Lawrence
Dec 3, 2011·BMC Genomics·Chunguang DuHugo K Dooner
Jul 30, 2004·BMC Plant Biology·Bradley J TillSteven Henikoff
Oct 13, 2006·PLoS Genetics·Jianbo ZhangThomas Peterson
Aug 28, 2007·Genetics·Liza J ConradThomas P Brutnell
Aug 20, 2009·Yi chuan = Hereditas·Shao-Hua ZengYing Wang
Oct 15, 2010·Yi chuan = Hereditas·Guan-Qing HuangHua-Sun Huang
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Clifford F Weil
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Huihua Fu, Hugo K Dooner
Feb 6, 2008·Nature Reviews. Genetics·Héctor Candela, Sarah Hake
May 28, 2008·Cytogenetic and Genome Research·G JenkinsR N Jones
Sep 20, 2005·The Plant Journal : for Cell and Molecular Biology·Donald R McCartyL Curtis Hannah
Jul 1, 2004·The Plant Journal : for Cell and Molecular Biology·Chul Min KimChang-deok Han
Dec 17, 2003·The Plant Journal : for Cell and Molecular Biology·Zhengrong Ma, Hugo K Dooner
Dec 24, 2003·The Plant Journal : for Cell and Molecular Biology·Tatiana KolesnikSrinivasan Ramachandran
Aug 8, 2006·The Plant Journal : for Cell and Molecular Biology·Tiehan ZhaoThomas Koprek
Jan 27, 2015·Annual Review of Plant Biology·Thomas P BrutnellJohn P Vogel
Jan 22, 2005·Plant & Cell Physiology·Gynheung AnSeong-Ryong Kim

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