PMID: 9429669Jan 16, 1998Paper

Use of thiocyanate elution to estimate relative avidity of allergen specific IgE antibodies

Allergy and Asthma Proceedings
M P PoirierW K Dolen

Abstract

Although several methods for estimating avidity of antigen-antibody reactions are available, most are impractical for the study of human IgE antibodies because of a requirement for pure allergen and antibody in relatively large amounts. To determine the relative avidity of specific IgE antibodies for Dermatophagoides pteronyssinus allergens, seven concentrations of the chaotropic thiocyanate ion were used to disrupt epitope-antibody binding in a specific IgE immunoassay system, using sera from 16 allergic patients with marked skin test reactivity to a standardized D. pteronyssinus extract. Relative avidity, the molarity of thiocyanate required to produce a 50% decrement in binding, ranged from 0.29-3.1. Within assay coefficient of variation (CV) was 9.9% and between assay CV was 13%. D. pteronyssinus specific IgE levels ranged from 0.66-141 kUa/L, not correlating with relative avidity (rho = -0.12). Thiocyanate elution appears to be a useful method for estimating relative avidity of specific IgE antibodies for the myriad epitopes of the allergenic proteins in an allergen extract. It could be used to study the immunochemistry of specific IgE assays; avidity maturation in allergen immunotherapy and in asymptomatic but sensitized ...Continue Reading

Citations

Feb 27, 2007·The Journal of Allergy and Clinical Immunology·Mübeccel Akdis, Cezmi A Akdis
Nov 16, 2007·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Fatima El-KhoulyJonathan O'Brien Hourihane
Nov 9, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·H MitaK Akiyama
Mar 7, 2021·The Journal of Allergy and Clinical Immunology·Oliver HemmingsAlexandra F Santos

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