PMID: 11920667Mar 29, 2002Paper

Use of Ti-coated replicas to investigate the effects on fibroblast shape of surfaces with varying roughness and constant chemical composition

Journal of Biomedical Materials Research
Marco WielandD M Brunette

Abstract

A two-stage replica technique with a subsequent titanium (Ti)-coating treatment was used to faithfully replicate topographies of polished, acid-etched, machined-like, finely blasted, coarsely blasted, coarsely blasted and acid-etched, and Ti plasma-sprayed Ti surfaces. The replicas were used to study the influence of different rough surface topographies on the response of human fibroblasts in vitro under conditions of constant surface chemistry for all surfaces. The surface topographies of the replicas were characterized using non-contact laser profilometry, scanning electron microscopy (SEM), and stereo-SEM, whereas surface chemistry was examined using X-ray photoelectron spectroscopy. Fibroblasts were trypsinized and plated onto the Ti-coated epoxy-resin replica surfaces for 24 h and observed with SEM. Fluorescein-5-thiosemicarbazide was used to stain the cell components including cell membrane, and the stained cells were optically sectioned using epifluorescent microscopy. The optical sections were computationally reconstructed to obtain three-dimensional images and cell volume and cell thickness determined. The different surface topographies were found to alter cell thickness and cell morphology. However, cell volume as com...Continue Reading

References

Jun 1, 1978·Nature·J Folkman, A Moscona
Jan 1, 1992·The International Journal of Oral & Maxillofacial Implants·K T BowersC M Michaels
Apr 1, 1992·Journal of Biomedical Materials Research·B ChehroudiD M Brunette
May 1, 1991·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A Ben-Ze'ev
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·F M WattC H O'Neill
Oct 1, 1989·Current Opinion in Cell Biology·J A McDonald
Aug 1, 1971·Experimental Cell Research·M AbercrombieS M Pegrum
Mar 1, 1995·Journal of Biomedical Materials Research·B W CallenJ E Davies
Mar 1, 1995·Journal of Biomedical Materials Research·B ChehroudiD M Brunette
Nov 1, 1994·Journal of Cellular Biochemistry·Z Schwartz, B D Boyan
Jan 1, 1997·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·R T GibersonR W Nordhausen
May 30, 1997·Science·C S ChenD E Ingber
Sep 7, 2004·Journal of Materials Science. Materials in Medicine·C SittigP H Vallotton

❮ Previous
Next ❯

Citations

Apr 20, 2011·Journal of Orofacial Orthopedics = Fortschritte Der Kieferorthopädie : Organ/official Journal Deutsche Gesellschaft Für Kieferorthopädie·Sandra HornikelHeiner Wehrbein
Dec 5, 2006·Journal of Materials Science. Materials in Medicine·F S Magdon IsmailA Bendavid
Feb 27, 2007·Journal of Materials Science. Materials in Medicine·D O MeredithR G Richards
May 29, 2010·Journal of Materials Science. Materials in Medicine·Alistair ForbesElizabeth James
May 28, 2013·Materials Science & Engineering. C, Materials for Biological Applications·N YangZ Du
Oct 21, 2004·Biomedizinische Technik. Biomedical Engineering·U BoudriotJ H Wendorff
Apr 25, 2003·Journal of Periodontology·Maristella Di CarmineAdriano Piattelli
Apr 8, 2004·Journal of Periodontology·Stefano GuizzardiRenato Scandroglio
Aug 25, 2007·Nanomedicine·Martin SchulerSamuele G P Tosatti
Apr 5, 2011·Materials Science & Engineering. C, Materials for Biological Applications·Aparna Raman, Ellen S Gawalt
Jan 8, 2008·Experimental Cell Research·Hugh KimChristopher A McCulloch
Feb 8, 2008·Journal of Biomedical Materials Research. Part a·Martin SchulerSamuele G P Tosatti
Feb 22, 2013·Journal of Biomedical Materials Research. Part a·Salem GhrebiDonald M Brunette
Feb 22, 2013·Journal of Biomedical Materials Research. Part a·Katrin A BarthDonald M Brunette
Aug 10, 2010·Journal of Biomedical Materials Research. Part a·S GiljeanK Anselme
May 24, 2012·Journal of Biomedical Materials Research. Part a·Mohammad Sayem MozumderHiran Perinpanayagam
May 23, 2012·Journal of Biomedical Materials Research. Part a·Humberto Osvaldo Schwartz-FilhoRosemary Adriana Chiérici Marcantonio
Apr 16, 2014·Journal of Biomedical Materials Research. Part a·Min LaiBarbara D Boyan
Jul 18, 2009·Journal of Biomedical Materials Research. Part a·Babak ChehroudiDonald Maxwell Brunette
Aug 22, 2014·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Morgan M StantonChristopher R Lambert
Jul 8, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Clément V M CremmelNicholas D Spencer
Nov 16, 2014·Tissue Engineering. Part C, Methods·Lara Yildirimer, Alexander M Seifalian
Jul 9, 2016·Nanomedicine : Nanotechnology, Biology, and Medicine·Alexey KlymovX Frank Walboomers
Oct 23, 2016·Dental Materials : Official Publication of the Academy of Dental Materials·Oleh AndrukhovAndreas Schedle
Apr 23, 2016·Journal of Biomedical Materials Research. Part a·Haisle MoonDonald M Brunette
May 13, 2017·Journal of Biomedical Materials Research. Part a·F KianoushD M Brunette
Apr 27, 2010·Journal of Biomaterials Applications·Mohammad Sayem MozumderHiran Perinpanayagam
Jul 1, 2004·Journal of Biomedical Materials Research. Part a·Ali K RefaiJ Douglas Waterfield
Aug 15, 2003·Journal of Biomedical Materials Research. Part a·J Carson MeredithEric J Amis
Jan 25, 2007·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Serene CheungMark Filiaggi
Jun 1, 2005·Journal of Biomedical Materials Research. Part a·B BaharlooD M Brunette
May 11, 2011·Biomedical Materials·Mohammad Sayem MozumderHiran Perinpanayagam
Feb 4, 2011·Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine·A PoncheK Anselme
Nov 15, 2014·Journal of Biomaterials Applications·Benjamin MüllerHanna Tiainen
Apr 30, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Doris M SporiNicholas D Spencer
May 4, 2021·International Journal of Implant Dentistry·Chu-Nan ZhangHong-Chang Lai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.