Use of transepithelial electrical resistance in the study of pentachlorophenol toxicity

Toxicology in Vitro : an International Journal Published in Association with BIBRA
G VelardeJ M Porcher

Abstract

The toxicity of pentachlorophenol (PCP), a polluting substance believed to exert a narcotic effect, was assayed using the Caco-2 cell line as a model. In order to assess this toxicity as fully as possible, several viability tests, each examining different endpoints, have been used. Neutral red uptake was found to be more sensitive to PCP than MTT and Alamar Blue tests. Transepithelial electrical resistance (TEER) was shown to be the most sensitive to PCP at concentrations and exposure times where the Alamar Blue, LDH leakage and Blue Dextran passage did not evidence any effect. Blue Dextran passage and optical microscopy revealed cellular detachment at concentrations where LDH and Alamar Blue showed little or no cytotoxicity. Thus, PCP seems to affect the integrity of the intestinal barrier at levels where no cytotoxicity is seen. Our results support the notion that TEER can be used as a very sensitive method for evaluating membrane-perturbing toxicants.

References

Feb 1, 1985·Toxicology Letters·E Borenfreund, J A Puerner
Jul 1, 1996·Ecotoxicology and Environmental Safety·J L HuglaJ P Thome
Feb 1, 1996·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A RossiM L Scarino
Aug 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A NaraiM Shimizu
Aug 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·G VelardeJ M Porcher
Jul 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·F Zucco
Jul 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·O VincentiniF Zucco

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Citations

Jul 14, 2005·European Journal of Drug Metabolism and Pharmacokinetics·F MathieuC Lartigue
Mar 30, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Sue ChoiYasuyuki Sakai
Jan 23, 2015·Scientific Reports·Lenke HorváthBarbara Rothen-Rutishauser
Jul 19, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·José Moisés LaparraRosaura Farré
Feb 3, 2016·Archives of Environmental Contamination and Toxicology·Britta EklundAnn-Kristin Eriksson Wiklund

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