Use of Trichomonas vaginalis clinical isolates to evaluate correlation of gene expression and metronidazole resistance

The Journal of Parasitology
J R MeadWilliam E Secor

Abstract

We investigated whether variations in gene expression of enzymes associated with anaerobic resistance of laboratory-derived strains of Trichomonas vaginalis could be detected in a group of 28 clinical isolates with variations in metronidazole sensitivity. We compared isolates by real-time PCR because this method allows for highly sensitive quantification of mRNA and for evaluation of several genes simultaneously. We found that PFOR gene A mRNA levels were highly correlated with PFOR gene B levels, as well as the D subunit of malic enzyme and ferrodoxin. Ferrodoxin mRNA expression was also significantly correlated with that of malic enzyme and hydrogenase. However, when we evaluated relationships between these enzymes and resistance to metronidazole, we found no significant correlations between aerobic or anaerobic in vitro sensitivity to drug and mRNA levels of any of the enzymes tested. Similarly, using a Student's t-test, no significant differences in enzyme mRNA levels were observed between isolates separated by metronidazole resistance or susceptibility. The lack of correlation between gene expression and resistance or susceptibility could be the result of differences in expression at the protein level or because other bioc...Continue Reading

References

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Citations

Mar 13, 2013·Antimicrobial Agents and Chemotherapy·Natalia MalloJosé M Leiro
Dec 12, 2012·Trends in Parasitology·Melissa D ConradJane M Carlton
Jan 26, 2018·Parasitology Research·Ursula FürnkranzJulia Walochnik
Feb 22, 2012·Expert Review of Anti-infective Therapy·W Evan Secor

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Methods Mentioned

BETA
PCR

Software Mentioned

Basic Local Alignment Search Tool ( BLAST
Primer Express

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