PMID: 7520916May 1, 1994Paper

Use of TrpE fusion protein to identify antigenic domains within the BIV envelope protein

Journal of Virological Methods
P ChenC Wood

Abstract

Nine different recombinant clones spanning various regions of the bovine immunodeficiency-like virus (BIV) envelope gene open reading frame were generated. These clones span the entire external glycoprotein as well as the transmembrane glycoprotein region. These proteins were expressed as fusions to the TrpE protein in E. coli. The levels of recombinant protein expressed varied, some clones expressed enough protein that can be detected in a Coomassie blue-stained gel, whereas other proteins could only be detected by Western blot analyses. A recombinant env protein representing the extracellular domain of the env protein was detected by BIV-infected bovine sera. In addition, a 134 amino acid peptide which may represent a major immunoreactive epitope was identified. This peptide is located at the amino terminus of the transmembrane glycoprotein and was specifically recognized by all BIV-infected calf sera tested. The identification of this epitope and the use of recombinant envelope protein will enable us to develop a more effective screening test to study the epidemiology of BIV infection.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Dec 21, 1981·Nucleic Acids Research·C YanofskyA M Wu

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Citations

May 12, 2001·Veterinary Microbiology·P BarboniM E Collins
Mar 27, 1999·Protein Expression and Purification·D BetempsT Baron
Mar 5, 2020·Transboundary and Emerging Diseases·Víctor David González-FernándezHugo Ramírez Álvarez

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