Use of two real-time polymerase chain reactions (PCRs) to detect herpes simplex type 1 and 2-DNA after automated extraction of nucleic acid

Journal of Medical Virology
Catherine MengelleJ Izopet

Abstract

Herpes simplex virus infections may be diagnosed by several techniques, including conventional cell culture and the polymerase chain reaction (PCR). This prospective study compares the analytical performances and usefulness of an in-house real-time PCR method and the Light Cycler HSV (1/2) detection kit (Roche Diagnostics, Mannheim, Germany). The results of both PCRs were then compared to those obtained by conventional cell culture. A total of 313 samples were tested (70 dermal samples, 81 cerebrospinal fluids (CSF), 47 ocular, 42 anogenital, 34 throat swabs, and 33 oral samples, 3 whole blood, 2 biopsies, and 1 bronchoalveolar lavage). Samples for molecular assays were extracted twice with the MagNa Pure instrument (Roche Molecular Biochemicals, Mannheim, Germany) and tested blind in parallel by the two PCR methods. Most (226) samples were also examined by cell culture. Forty three samples were found positive by both PCRs, whereas 267 were negative. The HSV-1 and -2 typing of positive samples was identical. Three of the samples were positive in the in-house PCR and negative in the Light Cycler HSV (1/2) detection kit. There was no statistically significant difference between the two tests. Only one sample gave an invalid resul...Continue Reading

References

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Citations

May 2, 2006·Molecular Diagnosis & Therapy·Lara B Strick, Anna Wald
Jan 27, 2007·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Julian HodgsonMelvyn Smith
Dec 16, 2010·Acta Ophthalmologica·Vishal JhanjiRasik B Vajpayee
Sep 28, 2006·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A M Simoons-SmitC M Vandenbroucke-Grauls
Oct 3, 2014·Journal of Clinical Microbiology·Matthew J BinnickerCole L Irish
Aug 28, 2020·Eye·Daniel Sibley, Daniel F P Larkin
Mar 7, 2020·Frontiers in Neurology·Guillaume DorcetJérémie Pariente

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