Use of various common isolation media to evaluate the new VITEK 2 colorimetric GN Card for identification of Burkholderia pseudomallei

Journal of Clinical Microbiology
Peter LoweKirsty Lewis

Abstract

The use of automated systems in the modern microbiology laboratory is becoming commonplace as the pressure of cost containment impacts on staff resources. With increased international travel and threats of bioterrorism, recognition and accurate identification of organisms such as Burkholderia pseudomallei is important. In areas where this organism is endemic, identification is not usually problematic. This study evaluates the performance of the new VITEK 2 colorimetric GN card for the identification of this organism. A total of 103 previously identified clinical isolates were tested with the new card with isolates taken from MacConkey agar, Columbia horse blood agar, Columbia sheep blood agar, and Trypticase soy agar in order to determine identification performance and to see if there was any variability in results due to the agar. Columbia horse blood agar produced the highest rates of identification (81%), followed by Trypticase soy agar (78%), Columbia sheep blood agar (75%), and MacConkey agar (63%). There was considerable variability in some of the reactions obtained. Seven isolates failed to identify from any of the agars used. This study highlights issues with the identification of this organism with the new VITEK 2 GN c...Continue Reading

References

Nov 28, 2002·Journal of Clinical Microbiology·Peter LoweRobert Norton
Sep 15, 2004·Journal of Clinical Microbiology·Guido Funke, Pascale Funke-Kissling
Apr 9, 2005·Clinical and Diagnostic Laboratory Immunology·Vanaporn WuthiekanunSharon J Peacock
Apr 16, 2005·Clinical Microbiology Reviews·Allen C Cheng, Bart J Currie
May 6, 2005·Journal of Clinical Microbiology·Timothy J J InglisGerry Harnett

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Citations

Jan 16, 2009·Transactions of the Royal Society of Tropical Medicine and Hygiene·Rama Narayana DeepakElaine Phang
Jun 21, 2013·Journal of Clinical Microbiology·Yuwana PodinBart J Currie
Oct 25, 2011·Journal of Travel Medicine·Liv HesstvedtAnne Ma Dyrhol-Riise
Apr 22, 2015·International Journal of Environmental Research and Public Health·Feng YuWuling Chen
Jul 24, 2012·Journal of Medical Microbiology·Zhiyong ZongTaoyou Zhou
Jan 28, 2015·Emerging Infectious Diseases·Alex R HoffmasterHenry T Walke
Mar 29, 2014·Journal of Clinical Microbiology·Samuel MaloneyRobert Norton
Aug 31, 2006·Current Opinion in Infectious Diseases·Sharon J Peacock
Sep 14, 2007·Scandinavian Journal of Infectious Diseases·Jacob GiladDavid Schwartz
Apr 25, 2015·Experimental Biology and Medicine·Susanna K P LauPatrick C Y Woo
Nov 15, 2018·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Jin LiWeiping Lu
Mar 13, 2020·Clinical Microbiology Reviews·I GassiepR Norton

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