Use of western blot to study Microsporum canis antigenic proteins in canine dermatophytosis

Mycoses
Andrea PeanoMaria Grazia Gallo

Abstract

Western blotting was used to describe the Microsporum canis proteins with antigenic activity in dogs with dermatophytosis. Electrophoretic separation of whole fungal strain extract cultured from a cat was performed under denaturing conditions. The proteins were blotted onto nitrocellulose and probed with sera collected from 22 dogs with dermatophytosis (18 M. canis, 3 M. gypseum, 1 Trichophyton mentagrophytes; group A), 20 dogs with skin diseases other than dermatophytosis, and 22 dogs with no clinical cutaneous signs (group B, n = 42). Nine principal IgG-binding proteins with apparent molecular weights of 180, 144, 130, 120, 102, 96, 80, 68, and 48 kD were visualised on group A blots. For these proteins, serological cross-reactivity with different strains of M. canis may be indirectly confirmed, whereas additional proteins were found to react with sera from individual dogs. The proteins visualised in this study may represent diagnostic markers of dermatophyte infection. The proteins should be further evaluated for their role in the cellular immune response of dogs with dermatophytosis.

References

May 1, 1995·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·A H SparkesT J Gruffydd-Jones
Jul 1, 1995·The Veterinary Clinics of North America. Small Animal Practice·K A Moriello, D J DeBoer
Jan 1, 1996·Mycopathologia·F J CabañesG Castellá
Sep 1, 1996·Research in Veterinary Science·A H SparkesC R Stokes
Aug 28, 1998·Research in Immunology·J A Woodfolk, T A Platts-Mills
May 24, 2000·Peptides·V P Kurup, B Banerjee
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J A WoodfolkT A Platts-Mills
May 27, 2003·Journal of Feline Medicine and Surgery·K A MorielloM Fintelman
Jan 22, 2004·FEMS Immunology and Medical Microbiology·Sandy M VermoutBernard R Mignon
Feb 10, 2004·The Veterinary Record·M E GarciaJ L Blanco
Jan 18, 2005·Clinical Microbiology Reviews·Judith A Woodfolk
May 15, 2008·Mycopathologia·Sandro Rogerio Almeida
Aug 23, 2008·Journal of Medical Microbiology·Aline BaldoBernard Mignon
Feb 4, 2009·Acta Clinica Belgica·C Piérard-FranchimontP Quatresooz

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Citations

Jul 19, 2016·Veterinary Dermatology·Verena K Affolter

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