Use of X-ray micro computed tomography imaging to analyze the morphology of wheat grain through its development

Plant Methods
Thang Duong Quoc LeAnne-Laure Chateigner-Boutin

Abstract

Wheat is one of the most important staple source in the world for human consumption, animal feed and industrial raw materials. To deal with the global and increasing population demand, enhancing crop yield by increasing the final weight of individual grain is considered as a feasible solution. Morphometric analysis of wheat grain plays an important role in tracking and understanding developmental processes by assessing potential impacts on grains properties, size and shape that are major determinants of final grain weight. X-ray micro computed tomography (μCT) is a very powerful non-invasive imaging tool that is able to acquire 3D images of an individual grain, enabling to assess the morphology of wheat grain and of its different compartments. Our objective is to quantify changes of morphology during growth stages of wheat grain from 3D μCT images. 3D μCT images of wheat grains were acquired at various development stages ranging from 60 to 310 degree days after anthesis. We developed robust methods for the identification of outer and inner tissues within the grains, and the extraction of morphometric features using 3D μCT images. We also developed a specific workflow for the quantification of the shape of the grain crease. The ...Continue Reading

References

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Citations

Dec 15, 2020·Plant Phenomics : a Science Partner Journal·Weijuan HuFan Chen
Mar 30, 2021·Plant Science : an International Journal of Experimental Plant Biology·Anne-Laure Chateigner-BoutinFabienne Guillon
Apr 17, 2021·Frontiers in Plant Science·Jessica K Van HarsselaarStefan Gerth

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Methods Mentioned

BETA
light microscopy
confocal microscopy
imaging techniques
imaging
X-ray
light
dissection

Software Mentioned

FiJi
MATLAB

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