PMID: 7539923Jun 6, 1995Paper

Use of yeast artificial chromosomes (YACs) in studies of mammalian development: production of beta-globin locus YAC mice carrying human globin developmental mutants

Proceedings of the National Academy of Sciences of the United States of America
K R PetersonG Stamatoyannopoulos

Abstract

To test whether yeast artificial chromosomes (YACs) can be used in the investigation of mammalian development, we analyzed the phenotypes of transgenic mice carrying two types of beta-globin locus YAC developmental mutants: (i) mice carrying a G-->A transition at position -117 of the A gamma gene, which is responsible for the Greek A gamma form of hereditary persistence of fetal hemoglobin (HPFH), and (ii) beta-globin locus YAC transgenic lines carrying delta- and beta-globin gene deletions with 5' breakpoints similar to those of deletional HPFH and delta beta-thalassemia syndromes. The mice carrying the -117 A gamma G-->A mutation displayed a delayed gamma- to beta-globin gene switch and continued to express A gamma-globin chains in the adult stage of development as expected for carriers of Greek HPFH, indicating that the YAC/transgenic mouse system allows the analysis of the developmental role of cis-acting motifs. The analysis of mice carrying 3' deletions first provided evidence in support of the hypothesis that imported enhancers are responsible for the phenotypes of deletional HPFH and second indicated that autonomous silencing is the primary mechanism for turning off the gamma-globin genes in the adult. Collectively, our...Continue Reading

References

Mar 1, 1990·Genes & Development·R R BehringerT M Townes
Aug 1, 1991·Genes & Development·O HanscombeF Grosveld
Oct 7, 1988·Cell·O R Choi, J D Engel
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K R PetersonG Stamatoyannopoulos

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Citations

Jun 1, 1997·Molecular Biotechnology·E R Cameron
Feb 26, 2005·Experimental Hematology·George Stamatoyannopoulos
Aug 8, 1998·Trends in Cell Biology·C BouchardM Eilers
Jan 23, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Joanna Y ChinPeter M Glazer
Apr 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jian XuStuart H Orkin
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·S P McCormickS G Young
Oct 9, 2012·Nucleic Acids Research·Kenneth R PetersonSusanna Harju-Baker
Jul 21, 1998·Annals of the New York Academy of Sciences·K R PetersonG Stamatoyannopoulos
Jul 21, 1998·Annals of the New York Academy of Sciences·H RaftopoulosA Bank
Jun 20, 2006·Molecular and Cellular Biology·Man YuGeorge Stamatoyannopoulos
Oct 4, 2005·Molecular and Cellular Biology·Susanna HarjuKenneth R Peterson
Apr 16, 2005·Molecular and Cellular Biology·Akane OmoriKeiji Tanimoto
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·J R JessenS Lin
Mar 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Karin M L GaenslerLinda Flebbe-Rehwaldt
May 19, 2004·Proceedings of the National Academy of Sciences of the United States of America·Qiliang LiGeorge Stamatoyannopoulos
Nov 26, 2003·FEMS Microbiology Reviews·Natalay Kouprina, Vladimir Larionov
Nov 14, 2006·Molecular and Cellular Endocrinology·Brian P HermannLeslie L Heckert
Feb 10, 2006·Annals of the New York Academy of Sciences·Tatiana KarpovaLeslie L Heckert

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