Usefulness of a 16S rDNA real-time PCR to monitor neonatal sepsis and to assist in medical decision to discontinue antibiotics

The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians
Inês StranieriThelma Suely Okay

Abstract

To monitor the bacterial load in newborns with proven infections on the day of admission, 48 h and 7 days after treatment. Real-time PCR (qPCR) targeting the 16S rDNA. The study recruited 17 newborns and the bacterial load was in general low (<50 CFU/mL). In three of four deaths, the bacterial load values increased, and in 11 of the 13 survivors the values decreased until the third evaluation. Considering the extreme sensitivity and high negative predictive value of qPCR, this test could help to monitor the treatment of neonatal sepsis and to assist in medical decision to discontinue antibiotics.

References

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Jan 8, 2005·Pediatric Critical Care Medicine : a Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·Brahm GoldsteinUNKNOWN International Consensus Conference on Pediatric Sepsis
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Jun 15, 2014·Journal of Microbiological Methods·Elodie Barbau-PiednoirNadine Botteldoorn

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Citations

Apr 22, 2017·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Deepak SharmaPradeep Sharma
Nov 1, 2018·Revista Do Instituto De Medicina Tropical De São Paulo·Inês StranieriThelma Suely Okay

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Methods Mentioned

BETA
PCR

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