Usefulness of Chromogenic CromoCen® AGN agar medium for the identification of the genus Aeromonas: Assessment of faecal samples

Journal of Microbiological Methods
Ma Guadalupe Aguilera-ArreolaGraciela Castro-Escarpulli

Abstract

Selective screening media for the detection and identification of Aeromonas strains are needed to guide primary isolation procedures in the clinical laboratory. This study compared the selective CromoCen® AGN chromogenic agar medium for the detection and identification of Aeromonas strains that were isolated from various samples against the conventional selective agar media that are commonly used for the isolation of this organism in food, environmental and clinical samples. The Miles and Misra and ecometric methods were used to evaluate the microbiological performance of CromoCen® AGN chromogenic agar medium, which was shown to be satisfactory. A total of 14 reference Aeromonas strains, 44 wild strains and 106 clinical stool specimens were examined using both non-chromogenic selective agars that are commonly used for Aeromonas isolation and CromoCen® AGN agar. The latter exhibited 94.73% sensitivity and 100% specificity for the various samples. On CromoCen® AGN agar medium, Aeromonas formed colonies with light green, greenish and salmon pigments with or without a surrounding wide transparent zone (halo) of 2-3mm in diameter around the entire border. This medium is recommended for the isolation and potential identification of t...Continue Reading

References

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Dec 29, 2004·FEMS Microbiology Letters·Ma Guadalupe Aguilera-ArreolaGraciela Castro-Escarpulli
May 15, 2007·Canadian Journal of Microbiology·Jennifer R HuddlestonRandall M Jeter
Jan 13, 2010·Clinical Microbiology Reviews·J Michael Janda, Sharon L Abbott

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Citations

Sep 10, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Claudio R MartínezMaria E Cortés

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