Usefulness of Gastric Biopsy-Based Real-Time Polymerase Chain Reaction for the Diagnosis of Helicobacter pylori Infection in Children

Journal of Pediatric Gastroenterology and Nutrition
Nicolas KalachJosette Raymond

Abstract

The aim of the study was to assess the usefulness of gastric biopsy-based quantitative real-time polymerase chain reaction (qPCR) for the detection of Helicobacter pylori infection and the identification of clarithromycin-resistant strains in children. A gastric biopsy-based qPCR for the detection of H pylori infection and the identification of clarithromycin-resistant strains in children was evaluated in 62 children with infection and 341 children without infection. H pylori infection was considered by the "reference method" when culture was positive for both histology and rapid urease test (RUT). Results were compared with those obtained using the qPCR. The reference method versus H pylori qPCR positivity showed 95% confidence interval sensitivity 100% versus 100%, specificity 93.2% (86.9-99.4) versus 100%, positive predictive value 59.7% (47.4-71.9) versus 100%, negative predictive value 100% versus 100%, and, finally, test accuracy of 59.6% (47.3-71.8) versus 100%. Sixty-two children were found to be H pylori positive, based on the qPCR results. Among those, 31 children had both positive qPCR and culture with concordant antimicrobial susceptibility testing results, whereas 31 children had negative culture and positive qPCR....Continue Reading

References

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Citations

Jan 31, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Shuichi MiyamotoNaoya Sakamoto
Sep 24, 2015·Digestive Diseases and Sciences·Nathan S S Atkinson, Barbara Braden
Aug 18, 2016·Helicobacter·Andrea SustmannSibylle Koletzko
Aug 18, 2016·Helicobacter·Francis MégraudPhilippe Lehours
Jun 3, 2020·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Xiaohui WuQiuxue Cao
Sep 16, 2015·Helicobacter·Eleftheria Roma, Erasmo Miele
Feb 9, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Parisa SabbaghSoheil Ebrahimpour
Dec 5, 2017·Euroasian Journal of Hepato-gastroenterology·Gokben Ozbey, Alfizah Hanafiah
Jun 3, 2021·Journal of Clinical Medicine·Maria Pina Dore, Giovanni Mario Pes
Jun 30, 2021·Anales de pediatría : publicación oficial de la Asociación Española de Pediatría (A.E.P.)·Gonzalo Galicia PobletAna María Vegas Álvarez
Oct 14, 2021·Anales De Pediatría·Gonzalo Galicia PobletAna María Vegas Álvarez

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Methods Mentioned

BETA
biopsy
biopsies
PCR

Software Mentioned

- View System
Stat

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