Abstract
The aim of the study was to assess the usefulness of gastric biopsy-based quantitative real-time polymerase chain reaction (qPCR) for the detection of Helicobacter pylori infection and the identification of clarithromycin-resistant strains in children. A gastric biopsy-based qPCR for the detection of H pylori infection and the identification of clarithromycin-resistant strains in children was evaluated in 62 children with infection and 341 children without infection. H pylori infection was considered by the "reference method" when culture was positive for both histology and rapid urease test (RUT). Results were compared with those obtained using the qPCR. The reference method versus H pylori qPCR positivity showed 95% confidence interval sensitivity 100% versus 100%, specificity 93.2% (86.9-99.4) versus 100%, positive predictive value 59.7% (47.4-71.9) versus 100%, negative predictive value 100% versus 100%, and, finally, test accuracy of 59.6% (47.3-71.8) versus 100%. Sixty-two children were found to be H pylori positive, based on the qPCR results. Among those, 31 children had both positive qPCR and culture with concordant antimicrobial susceptibility testing results, whereas 31 children had negative culture and positive qPCR....Continue Reading
References
Oct 1, 1995·Journal of Clinical Microbiology·A P LageY Glupczynski
Oct 1, 1996·The American Journal of Surgical Pathology·M F DixonP Correa
Jun 13, 1998·Antimicrobial Agents and Chemotherapy·J RaymondC Dupont
Jan 7, 1999·Journal of Medical Microbiology·A TiveljungH J Monstein
Jan 9, 2003·Journal of Clinical Microbiology·Monica OleastroFrancis Mégraud
Jan 26, 2006·The American Journal of Surgical Pathology·Veronika ZsiklaGieri Cathomas
Feb 24, 2006·The American Journal of Gastroenterology·Javier P Gisbert, Víctor Abraira
Sep 20, 2006·Pediatrics·Robert W FrenckHenry F Frierson
Feb 21, 2007·Helicobacter·Josette RaymondNicolas Kalach
Aug 19, 2007·Journal of Clinical Gastroenterology·Ji Youn YooIn Sung Song
Dec 20, 2007·American Journal of Clinical Pathology·Jason WeissWilliam Watkin
Mar 13, 2008·European Journal of Gastroenterology & Hepatology·Bela MolnarZsolt Tulassay
Jul 21, 2009·European Journal of Pediatrics·Jeannette GuarnerSibylle Koletzko
Mar 23, 2010·Helicobacter·Josette RaymondChristophe Burucoa
Jun 15, 2010·Diagnostic Microbiology and Infectious Disease·Sonia AgudoManuel López-Brea
May 12, 2011·Journal of Pediatric Gastroenterology and Nutrition·Sibylle KoletzkoUNKNOWN H pylori Working Groups of ESPGHAN and NASPGHAN
Jun 1, 2011·PloS One·María José Ramírez-LázaroXavier Calvet
Jul 28, 2012·Journal of Clinical Microbiology·Jesús SaezGloria Royo
Aug 28, 2012·Diagnostic Microbiology and Infectious Disease·Sofía BeldaGloria Royo
Jan 15, 2013·BMC Gastroenterology·Zhiying OuSitang Gong
Sep 16, 2014·Journal of Pediatric Gastroenterology and Nutrition·Benjamin D Gold
Sep 16, 2014·Journal of Pediatric Gastroenterology and Nutrition·Midori MituiJason Y Park
Citations
Jan 31, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Shuichi MiyamotoNaoya Sakamoto
Sep 24, 2015·Digestive Diseases and Sciences·Nathan S S Atkinson, Barbara Braden
Aug 18, 2016·Helicobacter·Andrea SustmannSibylle Koletzko
Aug 18, 2016·Helicobacter·Francis MégraudPhilippe Lehours
Feb 7, 2018·Helicobacter·You-Hua WangYong Xie
Jun 3, 2020·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Xiaohui WuQiuxue Cao
Sep 16, 2015·Helicobacter·Eleftheria Roma, Erasmo Miele
Oct 6, 2018·United European Gastroenterology Journal·Thomas BazinDominique Lamarque
Feb 9, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Parisa SabbaghSoheil Ebrahimpour
May 23, 2018·Paediatric Drugs·Kallirroi KotileaPatrick Bontems
Jul 2, 2017·Acta Paediatrica·Nicolas KalachJosette Raymond
Dec 5, 2017·Euroasian Journal of Hepato-gastroenterology·Gokben Ozbey, Alfizah Hanafiah
Jun 3, 2021·Journal of Clinical Medicine·Maria Pina Dore, Giovanni Mario Pes
Jun 30, 2021·Anales de pediatría : publicación oficial de la Asociación Española de Pediatría (A.E.P.)·Gonzalo Galicia PobletAna María Vegas Álvarez
Jul 3, 2021·Journal of Clinical Medicine·Nabil GastliJosette Raymond
Aug 24, 2021·PloS One·Rawaa A HusseinYasin H Majeed
Oct 14, 2021·Anales De Pediatría·Gonzalo Galicia PobletAna María Vegas Álvarez