Usefulness of Immunohistochemistry for Microsatellite Instability Screening in Gastric Cancer

Gut and Liver
Yoon Sung BaeHyunki Kim

Abstract

The usefulness of immunohistochemistry to screen for the microsatellite instability (MSI) phenotype in gastric cancer remains unclear. Moreover, the prognostic value of MSI phenotypes in gastric cancer has been debated. The clinicopathologic parameters and survival outcomes of 203 MSI-high (MSI-H) and 261 microsatellite-stable (MSS) advanced gastric cancers (AGCs) were compared. Next, we compared the immunohistochemistry results for hMLH1 and hMSH2 with those of a polymerase chain reaction (PCR)-based method. Kaplan-Meier curves and a Cox proportional hazard regression model were used to conduct survival analyses. The MSI-H AGCs were correlated with older age (p<0.001), female gender (p=0.018), distal location (p<0.001), larger size (p=0.016), and intestinal type (p<0.001). Multivariate analysis revealed that the MSI-H phenotype was an independent favorable factor that was related to overall survival in patients with AGC (p<0.001). Compared with the PCR-based analysis, immunohistochemistry exhibited high sensitivity (91.1%) and specificity (98.5%) in the detection of MSI phenotypes. MSI-H gastric cancers have distinct clinicopathologic features and better prognoses, which suggests the necessity of MSI analysis in gastric cancer...Continue Reading

References

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Feb 22, 2011·Journal of Gastroenterology and Hepatology·Hyunki KimHoguen Kim

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Citations

Nov 2, 2019·Journal of Pathology and Translational Medicine·Yoonjin KwakHye Seung Lee
Jan 21, 2020·Cancer Cell International·Kai LiXiao Zhu
Mar 10, 2020·Annales de pathologie·Florence Renaud, Magali Svrcek

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Methods Mentioned

BETA
PCR

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SPSS

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