Usefulness of lymphokine-activated killer cells generated from bone marrow mononuclear cells for the purging of residual tumor cells in peripheral blood stem cell graft

Japanese Journal of Cancer Research : Gann
F Komatsu, M Kajiwara

Abstract

Lymphokine-activated killer (LAK) cells were generated from bone marrow mononuclear cells (BM), and the usefulness of the BM-LAK for purging of residual tumor cells in autologous peripheral blood stem cell (PBSC) graft was determined. The BM and peripheral blood lymphocytes (PBL) were obtained from the same bone marrow donors. The BM-LAK and PBL-LAK were generated by incubation with interleukin-2 for 7 days. The BM-LAK demonstrated higher killer activity against a lymphoma cell line Raji than the PBL-LAK. The BM-LAK also had a higher percentage of CD4-CD8-CD16+ cells than the PBL-LAK, which suggests that their high killer activity is related to these cells. The BM-LAK did not show any killer activity against the PBSC graft. However, they killed tumor cells which contaminated the PBSC graft, and in particular, killed chimeric bcr/abl messenger RNA-positive residual leukemic cells. These results suggest that the BM-LAK may be applicable for purging. As the BM-LAK possess higher killer activity than the PBL-LAK, they may be more useful than the PBL-LAK.

References

Sep 1, 1990·Japanese Journal of Cancer Research : Gann·Y IbayashiK Hashi
Jul 1, 1990·The Journal of Experimental Medicine·M R van den BrinkJ C Hiserodt
Jan 1, 1993·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·G ScambiaG Bonanno

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Citations

Sep 21, 2000·Critical Reviews in Oncology/hematology·N SabaA Keating
May 17, 2000·Journal of Hematotherapy & Stem Cell Research·L Berkahn
Aug 28, 2001·Journal of Hematotherapy & Stem Cell Research·G Maki

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