Usefulness of matrix-assisted laser desorption ionisation-time-of-flight mass spectrometry for identifying clinical Trichosporon isolates

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
J N de Almeida JúniorC Hennequin

Abstract

Trichosporon spp. have recently emerged as significant human pathogens. Identification of these species is important, both for epidemiological purposes and for therapeutic management, but conventional identification based on biochemical traits is hindered by the lack of updates to the species databases provided by the different commercial systems. In this study, 93 strains, or isolates, belonging to 16 Trichosporon species were subjected to both molecular identification using IGS1 gene sequencing and matrix-assisted laser desorption ionisation-time-of-flight (MALDI-TOF) analysis. Our results confirmed the limits of biochemical systems for identifying Trichosporon species, because only 27 (36%) of the isolates were correctly identified using them. Different protein extraction procedures were evaluated, revealing that incubation for 30 min with 70% formic acid yields the spectra with the highest scores. Among the six different reference spectra databases that were tested, a specific one composed of 18 reference strains plus seven clinical isolates allowed the correct identification of 67 of the 68 clinical isolates (98.5%). Although until recently it has been less widely applied to the basidiomycetous fungi, MALDI-TOF appears to ...Continue Reading

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Citations

Aug 1, 2015·PLoS Pathogens·Marçal MarinéGustavo Henrique Goldman
Nov 2, 2016·Frontiers in Microbiology·João N de Almeida Júnior, Christophe Hennequin
Apr 26, 2017·Frontiers in Cellular and Infection Microbiology·Cláudio Duarte-OliveiraCristina Cunha
Aug 31, 2017·World Journal of Microbiology & Biotechnology·Bamba SanataHennequin Christophe
Oct 30, 2019·Open Forum Infectious Diseases·Felix BongominPaschalis Vergidis
Oct 16, 2015·Journal of Fungi·Cosmeri RizzatoArianna Tavanti
Jun 21, 2019·BioMed Research International·Dequan ZhangRongya Yang
May 3, 2019·Skin Appendage Disorders·Alexandro BonifazLaura Castrejón

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