Usefulness of microsatellite typing in population genetic studies of Trypanosoma cruzi

Memórias do Instituto Oswaldo Cruz
A M MacedoRiva P Oliveira

Abstract

Through microsatellite analysis of 53 monoclonal populations of Trypanosoma cruzi, we found a remarkable degree of genetic polymorphism with no single multilocus genotype being observed more than once. The microsatellite profile proved to be stable during 70 generations of the CL Brener clone in culture. The microsatellite profiling presented also high diagnostic sensitivity since DNA amplifications could be achieved with less than 100 fg DNA, corresponding to half parasite total DNA content. Based on these technical attributes the microsatellite assay turns out to be an important tool for direct typing T. cruzi in biological samples. By using this approach we were able to type T. cruzi in feces of artificially infected bugs and in single cells sorted by FACS. The microsatellites have shown to be excellent markers for T. cruzi phylogenetic reconstruction. We used maximum parsimony based on the minimum number of mutational steps to build an unrooted Wagner network, which confirms previous conclusions based on the analysis of the D7 domain of the LSU rDNA gene that T. cruzi is composed by two major groups. We also obtained evidence that strains belonging to rRNA group 2 are subdivided into two genetically distant clusters, and th...Continue Reading

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Citations

May 26, 2004·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Jan M SchwenkenbecherGabriele Schönian
Jul 12, 2008·Transactions of the Royal Society of Tropical Medicine and Hygiene·Fernanda da Silva Manoel-CaetanoAna Elizabete Silva
Aug 23, 2003·Experimental Parasitology·Deila J FrancoConceição R S Machado
Jun 12, 2003·Molecular and Biochemical Parasitology·Aurélio PedrosoBianca Zingales
May 10, 2003·Microbes and Infection·Carlos A Buscaglia, Javier M Di Noia
Apr 10, 2009·Journal of Clinical Microbiology·Daniella Alchaar D'AvilaLúcia Maria Cunha Galvão
Feb 4, 2006·Journal of Clinical Microbiology·Sebastian OchsenreitherGabriele Schönian
Jul 18, 2012·PLoS Neglected Tropical Diseases·Helder Magno Silva ValadaresAndréa Mara Macedo
Jul 12, 2011·The Journal of Infectious Diseases·Darlene A PenaMário Steindel
Jul 15, 2015·Expert Review of Anti-infective Therapy·Louisa A MessengerCaryn Bern
Oct 13, 2009·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Gustave SimoTazoacha Asonganyi
Jun 9, 2009·International Journal for Parasitology·Julio César CarranzaBianca Zingales
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Aug 29, 2014·PLoS Neglected Tropical Diseases·Patricio DiosqueMatthew Yeo
Dec 8, 2016·Parasites & Vectors·Amaia Izeta-AlberdiJanine M Ramsey

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