Usefulness of multilocus sequence typing for characterization of clinical isolates of Candida albicans.

Journal of Clinical Microbiology
M-E BougnouxC d'Enfert

Abstract

Molecular characterization of Candida albicans isolates is essential for understanding the epidemiology of nosocomial infections caused by this yeast. Here, we investigated the potential value of multilocus sequence typing (MLST) for characterizing epidemiologically related or unrelated C. albicans strains of various clinical origins. Accordingly, we sequenced the internal regions (loci) of six selected housekeeping genes of 40 C. albicans clinical isolates and 2 reference strains. In all, 68 polymorphic nucleotide sites were identified, of which 65 were found to be heterozygous in at least one isolate. Ten to 24 different genotypes were observed at the different loci, resulting, when combined, in 39 unique genotype combinations or diploid sequence types (DSTs). When MLST was applied to 26 epidemiologically unrelated isolates and the 2 reference strains, it allowed the identification of 27 independent DSTs, thus demonstrating a discriminatory power of 99.7. Using multidimensional scaling together with the minimum spanning tree method to analyze interstrain relationships, we identified six groups of genetically related isolates on the basis of bootstrap values of greater than 900. Application of MLST to 14 epidemiologically rela...Continue Reading

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Citations

Mar 8, 2013·Applied Microbiology and Biotechnology·Iraz AlperSteve Labrie
Mar 31, 2004·Fungal Genetics and Biology : FG & B·Arianna TavantiDuncan J Shaw
Oct 15, 2003·Trends in Microbiology·Rachel Urwin, Martin C J Maiden
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Oct 12, 2011·Antimicrobial Agents and Chemotherapy·Keunsook K LeeCarol A Munro
Oct 24, 2012·Antimicrobial Agents and Chemotherapy·Louise A WalkerCarol A Munro
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Apr 10, 2007·Eukaryotic Cell·Frank C OddsChristophe d'Enfert
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Aug 19, 2011·Journal of Clinical Microbiology·Stephen J Salipante, Barry G Hall
Apr 17, 2009·Journal of Clinical Microbiology·Sebastiaan J van HalJock Harkness
Nov 8, 2003·Journal of Clinical Microbiology·M-E BougnouxF C Odds
Dec 10, 2003·Journal of Clinical Microbiology·Andrew R DodgsonAndrew J Fox
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Nov 8, 2005·Journal of Clinical Microbiology·Arianna TavantiFrank C Odds
Nov 8, 2005·Journal of Clinical Microbiology·Arianna TavantiFrank C Odds
Aug 6, 2005·Journal of Clinical Microbiology·Paula SampaioCélia Pais

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