Usefulness of Non-Invasive Fetal RHD Genotyping towards Immunoprophylaxis Optimization

Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie
Sebastián BlancoSandra Verónica Gallego

Abstract

Introduction: Since anti-D immunoprophylaxis given to D-negative pregnant women is a blood product, blood donations have an impact on the availability of prophylactic doses. The Pan American Health Organization reported, in June 2017, that less than half of blood donors are volunteers in Latin America and the Caribbean. In these countries, guidelines for use of anti-D prophylaxis are still controversial. The aim of this study was to demonstrate the convenience of a simple and cost-effectivene non-invasive prenatal diagnostic assay for anti-D prophylaxis optimization in multiethnic populations. Methods: Cell-free fetal DNA from plasma samples of D-negative pregnant women were analyzed by real-time PCR for simultaneous amplification of sequences of exons 5 and 10 of the RHD gene. Fetal RHD genotype was determined in 111 pregnant women. Neonates' phenotype was determined 72 h after birth. Results: Genotyping predicted fetal phenotype with 100% accuracy. Prenatal diagnosis showed 78% RHD-positive and 22% RHD-negative neonates. Conclusion: We demonstrated that, beyond the large genetic variation of the Rh system and the numerous D variants present in multiethnic groups, non-invasive fetal RHD genotyping using two sequences of the ge...Continue Reading

References

Dec 3, 2003·Transfusion·John Bowman
Apr 1, 2008·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·C Rouillac-Le SciellourJ-P Cartron
Mar 18, 2009·Biochemical Society Transactions·Deborah G MaddocksNeil D Avent
Jan 28, 2010·Blood Transfusion = Trasfusione Del Sangue·Giancarlo Maria LiumbrunoGiuliano Grazzini
Aug 10, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Manfred WieseUNKNOWN East German HCV Study Group
Mar 20, 2014·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Frederik Banch ClausenMorten Hanefeld Dziegiel
May 14, 2014·Transfusion·Carolina Trucco BoggioneCarlos M Cotorruelo
Aug 3, 2014·American Journal of Preventive Medicine·Shivani GuptaJohn M Inadomi
May 3, 2016·Blood Transfusion = Trasfusione Del Sangue·Carolina Trucco BoggioneCarlos M Cotorruelo
May 3, 2016·Blood Transfusion = Trasfusione Del Sangue·Frederik B Clausen

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