Usefulness of pressure-controlled ventilation at high inspiratory pressures to induce acute lung injury in mice

American Journal of Physiology. Lung Cellular and Molecular Physiology
Tobias EckleHolger K Eltzschig

Abstract

Acute lung injury (ALI), as occurs with prolonged mechanical ventilation, contributes to morbidity and mortality of critical illness, and studies on novel genetic or pharmacological targets are areas of intense investigation. Here, we systematically tested a murine model of ALI by using pressure-controlled ventilation to induce ventilator-induced lung injury. For this purpose, C57BL/6 or Sv129 mice were anesthetized and underwent tracheotomy followed by induction of ALI via mechanical ventilation. Mice were ventilated in a pressure-controlled setting at different inspiratory pressure levels (15-45 mbar) and over different times (0-90 min, 100% oxygen). As outcome parameters, we assessed pulmonary edema (wet-to-dry ratios), bronchoalveolar fluid albumin content, pulmonary myeloperoxidase activity, macrophage inflammatory protein-2, and pulmonary gas exchange. These studies revealed maximal differences in severity of lung injury between different mouse strains after 90 min of ventilation time at 45 mbar. Use of lower concentrations of inspired oxygen did not alter disease severity. Increases of CD73 transcript (5'-ectonucleotidase, pacemaker of extracellular adenosine production) or total pulmonary adenosine levels with mechanica...Continue Reading

References

Mar 1, 1994·American Journal of Respiratory and Critical Care Medicine·G R BernardR Spragg
Jun 16, 1999·Journal of Applied Physiology·C G TankersleyW Mitzner
May 4, 2000·The New England Journal of Medicine·L B Ware, M A Matthay
Nov 15, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·K G DavidsonI R Doyle
Mar 20, 2001·American Journal of Respiratory and Critical Care Medicine·H D HeldS Uhlig
Jan 16, 2002·American Journal of Respiratory and Critical Care Medicine·Ana VillagráLluis Blanch
Jan 21, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Olivier LesurDenis Lane
Jun 19, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Gisela LannigHans O Pörtner
Jun 14, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jörg ReutershanKlaus Ley
Aug 20, 2005·Nature Reviews. Immunology·Michail Sitkovsky, Dmitriy Lukashev
Oct 21, 2005·The New England Journal of Medicine·Gordon D RubenfeldLeonard D Hudson
Dec 7, 2005·The Journal of Experimental Medicine·Holger K EltzschigSean P Colgan
Dec 20, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·R VlahosG P Anderson
May 2, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jianguo XuMauricio Rojas
Jun 13, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Tobias EckleHolger K Eltzschig
Jul 4, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sandra PerkowskiMelpo Christofidou-Solomidou
Jul 25, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Minqing ZhaoVictor E Laubach
Aug 17, 2006·American Journal of Physiology. Renal Physiology·Almut GrenzHolger K Eltzschig
Aug 29, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Daisuke OkutaniMingyao Liu
Feb 3, 2007·Journal of the American Society of Nephrology : JASN·Almut GrenzHolger K Eltzschig
Mar 14, 2007·Circulation·Tobias EckleHolger K Eltzschig
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Phillip FactorSadis Matalon
Mar 27, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Erhe GaoWalter J Koch
Mar 31, 2007·Cell Death and Differentiation·B B Fredholm
Apr 7, 2007·Nature Medicine·James L Kreindler, Steven D Shapiro
Apr 20, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Almut GrenzHolger K Eltzschig
Jun 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tobias EckleHolger K Eltzschig
Jul 31, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kazutoshi HamanakaJames C Parker
Dec 7, 2007·Blood·Tobias EckleHolger K Eltzschig
Dec 29, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Joseph P Mizgerd, Shawn J Skerrett
Mar 1, 2008·Blood·Julio C Morote-GarciaHolger K Eltzschig
Mar 8, 2008·Gastroenterology·Hanna HartmannVolkhard A J Kempf
Apr 26, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Melanie L HartHolger K Eltzschig

❮ Previous
Next ❯

Citations

Nov 29, 2012·Mucosal Immunology·M KoeppenH K Eltzschig
Aug 20, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Janek HenesPeter Rosenberger
Jun 11, 2011·Current Opinion in Anaesthesiology·Michael KoeppenHolger K Eltzschig
Feb 11, 2012·Current Opinion in Critical Care·Almut GrenzHolger K Eltzschig
Jan 16, 2010·American Journal of Respiratory and Critical Care Medicine·Valbona MirakajPeter Rosenberger
Dec 10, 2009·American Journal of Respiratory Cell and Molecular Biology·Guillermo M AlbaicetaCarlos López-Otin
May 19, 2011·Journal of Visualized Experiments : JoVE·Michael KoeppenHolger K Eltzschig
May 5, 2011·Journal of Visualized Experiments : JoVE·Tobias EckleHolger Eltzschig
Nov 23, 2013·Journal of the American Society of Nephrology : JASN·Eunyoung TakAlmut Grenz
Oct 10, 2009·Physiology·Tobias EckleHolger K Eltzschig
Sep 22, 2017·Science Translational Medicine·Viola NeudeckerHolger K Eltzschig
Dec 27, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Valbona MirakajPeter Rosenberger
Apr 23, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tobias EckleHolger K Eltzschig
Oct 8, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jörg ReutershanHolger K Eltzschig
Mar 5, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Mengnan WangYouzhong An
Jan 7, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tobias EckleHolger K Eltzschig
May 16, 2015·Journal of Applied Physiology·Christine U VohwinkelHolger K Eltzschig
Mar 10, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Christine U VohwinkelHolger K Eltzschig
Feb 11, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kai SuGuochang Hu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.