Usefulness of real-time PCR assay targeting lipL32 gene for diagnosis of human leptospirosis in Uruguay

Journal of Infection in Developing Countries
Sabina GonzálezGustavo Varela

Abstract

Assays based on DNA amplification can provide information that contributes to the initial management of patients with leptospirosis. However, these have not been adopted in Uruguay. Our aim was to evaluate the performance of the lipL32 real-time PCR (qPCR) for diagnosis of leptospirosis. We analyzed by microscopic agglutination test (MAT) and lipL32 qPCR serum samples from 183 patients with suspected leptospirosis. To establish the analytical sensitivity of the qPCR, experimentally spiked samples with known amounts of Leptospira interrogans were analyzed. The analytical sensitivity of the qPCR was 102 leptospires/mL. In 98 patients MAT results were negative meanwhile 85 showed positive reactions, revealing acute infections. Twenty six acute-phase sera of these 85 patients showed a positive signal by qPCR (diagnostic sensitivity 30%). In these patients the average time between onset of symptoms and collection of the first sample was 8 days. In patients with negative results for qPCR and positive MAT results (n=59) the average interval between onset of symptoms and collection of the first sample was 13 days. The qPCR did not yield false positive results. The qPCR had a lower diagnostic sensitivity than MAT and a higher cost. Howe...Continue Reading

Citations

Aug 8, 2015·Microbial Pathogenesis·Bora Buyuktimkin, Milton H Saier
Jun 15, 2016·Microbial Pathogenesis·Bora Buyuktimkin, Milton H Saier
Sep 30, 2016·Revista Do Instituto De Medicina Tropical De São Paulo·Tülin Güven GökmenFatih Köksal
Aug 21, 2018·Tropical Doctor·Keerthivasan GurumurthyNiranjan Biswal
Aug 20, 2019·The Cochrane Database of Systematic Reviews·Bada YangMariska Mg Leeflang
Dec 22, 2017·Revista Do Instituto De Medicina Tropical De São Paulo·Paulina MenyGustavo Varela

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