Usefulness of techniques based on real time PCR for the identification of onychomycosis-causing species

Mycoses
Anissa Z HafirassouMaría José Buitrago

Abstract

Onychomycosis (OM) is a nail infection caused mainly by dermatophyte species but other species of yeast and moulds are frequently involved as well. Classical diagnosis has limitations thus empirical treatment is common. The usefulness of different real time PCR (RT-PCR) assays for identifying species causing OM was assessed in samples from seventy patients and fifteen controls. Conventional methods and four different RT-PCR assays were used: a panfungal, a pandermatophyte and two specific assays for detecting Candida spp. and Aspergillus spp. Fungal elements were visualised in 58% of the samples, and 54% of cultures were positive. Panfungal and pandermatophyte RT-PCR were positive in 28% and 60%, respectively, and the sensitivity relative to positive cultures was 47% and 90%. Candida spp. were detected in 76% of samples analysed and Aspergillus spp. in 60%. These species were also present in 80% of control cases. In conclusion, molecular techniques were useful but showed limitations. The panfungal assay showed a low sensitivity, the pandermatophyte assay was sensitive and specific but did not allow for differentiation among species of dermatophytes. Finally, the role of non-dermatophyte species detected by using specific RT-PCR...Continue Reading

References

Mar 26, 2003·The British Journal of Dermatology·D T RobertsUNKNOWN British Association of Dermatologists
May 10, 2006·Mycoses·Assya DjeridaneAomar Ammar-Khodja
Feb 20, 2007·Journal of the American Academy of Dermatology·Richard K ScherBoni E Elewski
Aug 9, 2011·Journal of the American Academy of Dermatology·Aditya K GuptaAntonella Tosti
Mar 8, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M J BuitragoM Cuenca-Estrella
Nov 29, 2013·Journal of the European Academy of Dermatology and Venereology : JEADV·B Sigurgeirsson, R Baran
Jun 6, 2014·PLoS Pathogens·Mahmoud Ghannoum, Nancy Isham
Jun 28, 2014·The Journal of Antimicrobial Chemotherapy·J FortúnM Cuenca-Estrella
Dec 3, 2014·Journal de mycologie médicale·D PetinataudM Machouart
Dec 31, 2015·JAMA Dermatology·Anar MikailovArash Mostaghimi
Apr 14, 2016·Seminars in Cutaneous Medicine and Surgery·Linda F Stein Gold
May 25, 2016·Clinics in Podiatric Medicine and Surgery·Tracey C Vlahovic
Aug 3, 2016·Mycopathologia·Julie Verrier, Michel Monod

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Citations

Sep 25, 2018·International Journal of Dermatology·Bilge Fettahl Oğlu KaramanVarol L Aksungur

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