Usefulness of the Non-conventional Caenorhabditis elegans Model to Assess Candida Virulence

Mycopathologia
Marcelo Ortega-RiverosElena Eraso

Abstract

Invasive candidiasis is caused mainly by Candida albicans, but other Candida species have increasing etiologies. These species show different virulence and susceptibility levels to antifungal drugs. The aims of this study were to evaluate the usefulness of the non-conventional model Caenorhabditis elegans to assess the in vivo virulence of seven different Candida species and to compare the virulence in vivo with the in vitro production of proteinases and phospholipases, hemolytic activity and biofilm development capacity. One culture collection strain of each of seven Candida species (C. albicans, Candida dubliniensis, Candida glabrata, Candida krusei, Candida metapsilosis, Candida orthopsilosis and Candida parapsilosis) was studied. A double mutant C. elegans AU37 strain (glp-4;sek-1) was infected with Candida by ingestion, and the analysis of nematode survival was performed in liquid medium every 24 h until 120 h. Candida establishes a persistent lethal infection in the C. elegans intestinal tract. C. albicans and C. krusei were the most pathogenic species, whereas C. dubliniensis infection showed the lowest mortality. C. albicans was the only species with phospholipase activity, was the greatest producer of aspartyl proteina...Continue Reading

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Sep 27, 2019·Medical Microbiology and Immunology·Moses MadendeCarolina H Pohl
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Sep 11, 2021·Medical Mycology·Si Jie LimSiti Nurbaya Oslan

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Methods Mentioned

BETA
Infection
Assay

Software Mentioned

SPSS
Stata

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