Abstract
In order to develop tools for an early serodiagnosis of Plasmodium falciparum infection, we evaluated the usefulness of P. falciparum liver stage antigen-3 (LSA-3) as a serodiagnostic antigen. A portion of LSA-3 gene was cloned, and its recombinant protein (rLSA-3) was expressed in Escherichia coli and purified by column chromatography. The purified rLSA-3 and 120 test blood/serum samples collected from inhabitants in malaria-endemic areas of Mandalay, Myanmar were used for this study. In microscopic examinations of blood samples, P. falciparum positive rate was 39.1% (47/120) in thin smear trials, and 33.3% (40/120) in thick smear trials. Although the positive rate associated with the rLSA-3 (30.8%) was lower than that of the blood stage antigens (70.8%), rLSA-3 based enzyme-linked immunosorbent assay could detect 12 seropositive cases (10.0%), in which blood stage antigens were not detected. These results indicate that the LSA-3 is a useful antigen for an early serodiagnosis of P. falciparum infection.
References
Sep 10, 1987·Nature·C Guerin-MarchandG Langsley
Dec 1, 1994·The Korean Journal of Parasitology·S Y ChoH K Shin
Sep 1, 1994·The Korean Journal of Parasitology·I H ChaiJ Y Chai
Dec 22, 1999·Infection and Immunity·S K JoshiV S Chauhan
Dec 22, 1999·Infection and Immunity·M AidooA V Hill
Nov 14, 2000·Nature Medicine·P DaubersiesL B Mohamed
Feb 13, 2001·Infection and Immunity·J P SauzetP Druilhe
Apr 27, 2001·Trends in Parasitology·J D KurtisP E Duffy
Jun 27, 2001·The American Journal of Tropical Medicine and Hygiene·J G Breman
Jul 13, 2001·European Journal of Immunology·B L PerlazaP Druilhe
Aug 23, 2001·The Journal of Infectious Diseases·T E Wellems, C V Plowe
Jan 5, 2002·Trends in Parasitology·R W SnowK Marsh
Mar 15, 2003·Immunologic Research·Andrew W Taylor-Robinson
Apr 11, 2003·Lancet·Cally RoperBrian Sharp
Oct 27, 2004·Antimicrobial Agents and Chemotherapy·J Kevin Baird
Mar 1, 2005·Pharmacological Reviews·Aric Gregson, Christopher V Plowe
Citations
Sep 11, 2007·Parasitology Research·Eva M MoyanoAgustín Benito
Aug 9, 2011·Malaria Journal·Tong-Soo KimHyeong-Woo Lee
Oct 31, 2009·Malaria Journal·Eric Prieur, Pierre Druilhe
Nov 9, 2011·Parasites & Vectors·Jean Biram SarrFranck Remoue
Feb 14, 2014·Parasites & Vectors·Youn-Kyoung GooJung-Yeon Kim
Oct 3, 2008·The Korean Journal of Parasitology·Hyeong-Woo LeeTong-Soo Kim
Jan 22, 2008·World Journal of Gastroenterology : WJG·Natthawut KaewpitoonBanchob Sripa