Usefulness of zebrafish in evaluating drug-induced teratogenicity in cardiovascular system

Drug and Chemical Toxicology
Ryo WatanabeKazuhiko Mori

Abstract

To confirm the usefulness of zebrafish for evaluating the teratogenic potential of drug candidates, the effect of O-ethylhydroxylamine hydrochloride (OHY), which induces mutagenesis by methylation, was evaluated in teratogenicity studies in rats and zebrafish. In the rat teratogenicity study, OHY-induced cardiovascular malformations such as increased abnormal vascular structures and ventricular septal defects. In the teratogenicity study using zebrafish-injected microspheres and green fluorescent protein-expressing Tg zebrafish (flk1:EGFP), OHY exposure was associated with the loss or malformation of the mandibular arch, opercular artery, and fourth branchial arch. These results suggested that OHY-induced external malformations in zebrafish eleutheroembryos adequately reflect OHY's teratogenicity in rat fetuses. Moreover, the zebrafish teratogenicity study incorporating vascular morphological examinations, including those of blood vessels in the heart, head and trunk, is an easy and reliable screening method to detect potential drug-induced teratogenicity and phenotypic characteristics.

References

Jan 1, 1989·Anatomy and Embryology·M C de RuiterR E Poelmann
Jul 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C B KimmelT F Schilling
Aug 9, 2002·Developmental Biology·Nathan D Lawson, Brant M Weinstein
Jan 13, 2006·Nature Chemical Biology·C Geoffrey BurnsMark C Fishman
Jul 1, 2008·Journal of Pharmacological and Toxicological Methods·Stéphane BerghmansAngeleen Fleming
Aug 15, 2008·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Robert ChapinGeneviève Van Maele-Fabry
Mar 13, 2010·Science·Takumi ItoHiroshi Handa
Jul 8, 2010·Current Biology : CB·Atsuo IidaAtsuko Sehara-Fujisawa
Aug 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·G ScholzH Spielmann
Sep 14, 2010·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Julieta M Panzica-KellyKaren A Augustine-Rauch
Sep 1, 1994·Trends in Cardiovascular Medicine·D Y Stainier, M C Fishman
May 23, 2014·The Journal of Toxicological Sciences·Akihito YamashitaHitoshi Funabashi
Nov 22, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Nishad JayasundaraRichard T Di Giulio
Feb 18, 2015·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Hsunhui YangLouise Lanoue
Sep 18, 2015·Frontiers in Cellular Neuroscience·Marco MorschRoger Chung

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

American Journal of Medical Genetics. Supplement
R MatsuokaB Nadal-Ginard
American Journal of Medical Genetics. Part a
Elizabeth A DuffyLisa A Schimmenti
American Journal of Obstetrics and Gynecology
P G PrydeD B Cotton
© 2022 Meta ULC. All rights reserved