Using a GFP-gene fusion technique to study the cell cycle-dependent distribution of calmodulin in living cells

Science in China. Series C, Life Sciences
C LiD Zhang

Abstract

In this study, a green fluorescent protein (GFP)-calmodulin (CaM) fusion gene method was used to examine the distribution of calmodulin during various stages of cell cycle. First, it was found that the distribution of CaM in living cells changes with the cell cycle. CaM was found mainly in the cytoplasm during G1 phase. It began to move into the nucleus when the cell entered S phase. At G2 phase, CaM became more concentrated in the nucleus than in cytoplasm. Second, the accumulation of CaM in the nucleus during G2 phase appeared to be related to the onset of mitosis, since inhibiting the activation of CaM at this stage resulted in blocking the nuclear membrane breakdown and chromatin condensation. Finally, after the cell entered mitosis, a high concentration of CaM was found at the polar regions of the mitotic spindle. At this time, inhibiting the activity of CaM would cause a dismption of the spindle structure. The relationship between the stage-specific distribution of CaM and its function in regulating the progression of cell cycle was discussed.

References

Feb 1, 1992·Current Opinion in Cell Biology·L L Satterwhite, T D Pollard
Apr 17, 1991·Biochimica Et Biophysica Acta·D C ChangB L Maxwell
Nov 1, 1990·The Journal of Cell Biology·C BaitingerR A Steinhardt
Jun 20, 1994·FEBS Letters·A R Means
Nov 1, 1995·Trends in Biochemical Sciences·A B CubittR Y Tsien

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Citations

May 15, 2012·Micron : the International Research and Review Journal for Microscopy·Marcus HezelFaramarz Dehghani
Nov 6, 2013·Biochimica Et Biophysica Acta·Martin W Berchtold, Antonio Villalobo
May 30, 2019·Hormone Molecular Biology and Clinical Investigation·Kian Chung ChokSoi Moi Chye
Apr 19, 2012·Human Molecular Genetics·Jack-Christophe CossecMarie-Claude Potier
Dec 12, 2012·Cellular Microbiology·Emanuel Martín CampoyMaría Isabel Colombo
Jun 15, 2016·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Ramsey N MajzoubCyrus R Safinya
Sep 17, 2013·PloS One·Janelle L HarrisMartin F Lavin
Oct 16, 2013·Journal of Experimental Botany·Marion C HoepflingerIlse Foissner

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