Using a kinase/phosphatase switch to regulate a supramolecular hydrogel and forming the supramolecular hydrogel in vivo

Journal of the American Chemical Society
Zhimou YangBing Xu

Abstract

We have designed and synthesized a new hydrogelator Nap-FFGEY (1), which forms a supramolecular hydrogel. A kinase/phosphatase switch is used to control the phosphorylation and dephosphorylation of the hydrogelator and to regulate the formation of supramolecular hydrogels. Adding a kinase to the hydrogel induces a gel-sol phase transition in the presence of adenosine triphosphates (ATP) because the tyrosine residue is converted into tyrosine phosphate by the kinase to give a more hydrophilic molecule of Nap-FFGEY-P(O)(OH)(2) (2); treating the resulting solution with a phosphatase transforms 2 back to 1 and restores the hydrogel. Electron micrographs of the hydrogels indicate that 1 self-assembles into nanofibers. Subcutaneous injection of 2 in mice shows that 80.5 +/- 1.2% of 2 turns into 1 and results in the formation of the supramolecular hydrogel of 1 in vivo. This simple biomimetic approach for regulating the states of supramolecular hydrogels promises a new way to design and construct biomaterials.

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Citations

Jul 9, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Ye ZhangBing Xu
Aug 6, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Derek M RyanBradley L Nilsson
May 24, 2012·ACS Nano·Naohiro KametaToshimi Shimizu
Aug 30, 2012·Nature Communications·Yuan GaoBing Xu
Mar 26, 2008·Chemical Society Reviews·Rein V Ulijn, Andrew M Smith
Dec 6, 2006·Chemical Communications : Chem Comm·Gaolin LiangBing Xu
Feb 28, 2007·Chemical Communications : Chem Comm·Qigang WangBing Xu
Oct 11, 2007·Chemical Communications : Chem Comm·Gaolin LiangBing Xu
Nov 21, 2008·Chemical Society Reviews·Parthasarathi Dastidar
Jan 1, 2009·Faraday Discussions·Apurba K DasRein V Ulijn
Apr 28, 2010·Chemical Society Reviews·Congqi Yan, Darrin J Pochan
Apr 29, 2010·Chemical Communications : Chem Comm·Malgorzata BroncelBeate Koksch
Apr 2, 2010·Chemical Communications : Chem Comm·Minh Khanh Nguyen, Doo Sung Lee
Jan 25, 2011·Chemical Communications : Chem Comm·Hyejin LeeJong Hwa Jung
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