Using a novel "Integrated Biomarker Proteomic" index to assess the effects of freshwater pollutants in European eel peripheral blood mononuclear cells

Journal of Proteomics
Kathleen RolandFrédéric Silvestre

Abstract

Using proteomic data as biomarkers of environmental pollution has the potential to be of a great interest in ecological risk assessment as they constitute early warning indicators of ecologically relevant effects on biological systems. To develop such specific and sensitive biomarkers, the use of a set of proteins is required and the identification of protein expression signatures (PES) may reflect the exposure to specific classes of pollutants. Using 2D-DIGE (Differential in Gel Electrophoresis) methodology, this study aimed at identifying specific PES on European eel (Anguilla anguilla) peripheral blood mononuclear cells (PBMC) after 48 h in vitro exposure to two sublethal concentrations of dichlorodiphenyltrichloroethane (p,p'-DDT) (10 μg/L and 1mg/L) or cadmium (Cd) (1 μg/L and 100 μg/L). The present results have been supplemented with data of a first in vitro study on cells exposed to perfluorooctane sulfonate (PFOS) (10 μg/L and 1mg/L). A total of thirty-four protein spots, belonging to 18 different identified proteins found in all conditions, have been selected as possible biomarkers to develop a synthetic Integrated Biomarker Proteomic (IBP) index. IBP follows a dose-response relationship with higher values at the highe...Continue Reading

References

Oct 13, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M J van HemertG P van Heusden
Nov 16, 2001·The Journal of Biological Chemistry·Guri Tzivion, Joseph Avruch
Feb 12, 2002·Environmental Health Perspectives·Vladimir TurusovLorenzo Tomatis
Jun 1, 2002·Environmental Science & Technology·Tamara S GallowayMichael H Depledge
Jun 19, 2002·Environmental Toxicology and Chemistry·Benoit Beliaeff, Thierry Burgeot
Aug 28, 2002·Biochemical Society Transactions·S C MastersH Fu
Oct 5, 2002·Chemosphere·Kurunthachalam KannanJohn P Giesy
Nov 1, 2002·Marine Environmental Research·Brian P BradleyJessica C Meiller
Nov 6, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A Gómez-Mendikute, M P Cajaraville
Nov 25, 2003·Analytical Chemistry·Alexey I NesvizhskiiRuedi Aebersold
Jun 5, 2004·Marine Environmental Research·G M WatsonT S Galloway
Feb 17, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M DavorenC Mothersill
Jan 10, 2006·Bulletin of Environmental Contamination and Toxicology·M NawazG Krumschnabel
Apr 11, 2006·Molecular & Cellular Proteomics : MCP·Itxaso ApraizSusana Cristobal
Aug 26, 2006·Science·René P SchwarzenbachBernhard Wehrli
Oct 13, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Fengxia TanYuanan Lu
Oct 13, 2007·The EMBO Journal·Guy Tran Van Nhieu, Tina Izard
Aug 5, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·F Filipak NetoC A Oliveira Ribeiro
Apr 4, 2009·Toxicology and Applied Pharmacology·Gunnar F Nordberg
Apr 30, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A AramphongphanL Himakoun
May 29, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Xiongjie ShiBingsheng Zhou
May 30, 2009·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Amalia Vioque-FernándezJuan López-Barea
Sep 1, 2009·Aquatic Toxicology·Suze ChoraMaria João Bebianno
Oct 7, 2009·Ecotoxicology·Caroline Geeraerts, Claude Belpaire
Nov 27, 2009·The Science of the Total Environment·Jan WeberTerry Bidleman
Dec 3, 2009·Proteomics·Marco F L LemosAna C Esteves

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Citations

Sep 4, 2020·Environmental Pollution·M López-PedrousoJ R Aboal

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