Using alpha-helical coiled-coils to design nanostructured metalloporphyrin arrays.

Journal of the American Chemical Society
Karen A McAllisterWilliam F Degrado

Abstract

We have developed a computational design strategy based on the alpha-helical coiled-coil to generate modular peptide motifs capable of assembling into metalloporphyrin arrays of varying lengths. The current study highlights the extension of a two-metalloporphyrin array to a four-metalloporphyrin array through the incorporation of a coiled-coil repeat unit. Molecular dynamics simulations demonstrate that the initial design evolves rapidly to a stable structure with a small rmsd compared to the original model. Biophysical characterization reveals elongated proteins of the desired length, correct cofactor stoichiometry, and cofactor specificity. The successful extension of the two-porphyrin array demonstrates how this methodology serves as a foundation to create linear assemblies of organized electrically and optically responsive cofactors.

References

Feb 15, 1987·Analytical Biochemistry·E A Berry, B L Trumpower
Jun 29, 2000·Annual Review of Biochemistry·W F DeGradoA Lombardi
Sep 5, 2001·Journal of Molecular Biology·B NorthW F DeGrado
Feb 3, 2005·Journal of the American Chemical Society·Frank V CochranWilliam F DeGrado
Apr 30, 1998·The Journal of Physical Chemistry. B·A D MacKerellM Karplus

❮ Previous
Next ❯

Citations

Sep 3, 2011·Biochemistry·Betty P TsangRobert Fairman
Sep 2, 2010·Biomacromolecules·Daniil V ZaytsevMichael Y Ogawa
Oct 28, 2011·Biomacromolecules·Brian J Pepe-MooneyRobert Fairman
Aug 25, 2012·Journal of the American Chemical Society·H Christopher FrySamuel I Stupp
Aug 13, 2013·Journal of the American Chemical Society·H Christopher FryMichael J Therien
Apr 15, 2010·Journal of the American Chemical Society·Aaron W ReinkeAmy E Keating
Nov 27, 2008·The Journal of Organic Chemistry·Maria L Diss, Alan J Kennan
Jun 2, 2012·Science·Yen-Ting LaiTodd O Yeates
Jul 5, 2013·Nucleic Acids Research·Daniel B GowetskiJason D Kahn
Dec 7, 2010·Annual Review of Physical Chemistry·Ilan SamishJeffery G Saven
May 17, 2014·Current Opinion in Biotechnology·François Baneyx, James F Matthaei
Oct 16, 2010·Journal of the American Chemical Society·Ivan V KorendovychWilliam F Degrado
Jan 17, 2012·Structure·Jose Manuel Perez-Aguilar, Jeffery G Saven
May 6, 2011·Current Opinion in Colloid & Interface Science·Jeffery G Saven
Jun 5, 2013·Current Opinion in Structural Biology·Bryan S Der, Brian Kuhlman
Oct 10, 2015·Biochimica Et Biophysica Acta·Vikas NandaDror Noy
Nov 26, 2015·Chemistry, an Asian Journal·Louise N Slope, Anna F A Peacock
Jan 19, 2010·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Pingping YaoMinghua Liu
Feb 16, 2021·Chemical Society Reviews·Chenyan LvGuanghua Zhao
Oct 23, 2009·The Journal of Physical Chemistry. B·Darius Kuciauskas, Gregory A Caputo
Apr 21, 2009·Biomacromolecules·Bashkim KokonaRobert Fairman
Nov 13, 2010·The Journal of Physical Chemistry. B·Darius KuciauskasVidmantas Gulbinas
May 19, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Xiangyu Sun, Luhua Lai
Dec 2, 2014·Journal of the American Chemical Society·Anindya RoyGiovanna Ghirlanda

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.