Using approximate Bayesian computation to quantify cell-cell adhesion parameters in a cell migratory process

NPJ Systems Biology and Applications
Robert J H RossChristian A Yates

Abstract

In this work, we implement approximate Bayesian computational methods to improve the design of a wound-healing assay used to quantify cell-cell interactions. This is important as cell-cell interactions, such as adhesion and repulsion, have been shown to play a role in cell migration. Initially, we demonstrate with a model of an unrealistic experiment that we are able to identify model parameters that describe agent motility and adhesion, given we choose appropriate summary statistics for our model data. Following this, we replace our model of an unrealistic experiment with a model representative of a practically realisable experiment. We demonstrate that, given the current (and commonly used) experimental set-up, our model parameters cannot be accurately identified using approximate Bayesian computation methods. We compare new experimental designs through simulation, and show more accurate identification of model parameters is possible by expanding the size of the domain upon which the experiment is performed, as opposed to increasing the number of experimental replicates. The results presented in this work, therefore, describe time and cost-saving alterations for a commonly performed experiment for identifying cell motility pa...Continue Reading

References

Dec 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Paul MarjoramSimon Tavare
Nov 3, 2004·Nature Reviews. Genetics·Hiroaki Kitano
Dec 26, 2006·Journal of Theoretical Biology·Anna Q CaiBarry D Hughes
Jun 24, 2008·Journal of Theoretical Biology·Simeone MarinoDenise E Kirschner
Feb 11, 2009·Journal of the Royal Society, Interface·Tina ToniMichael P H Stumpf
Oct 5, 2010·Statistical Applications in Genetics and Molecular Biology·Matthew A Nunes, David J Balding
Aug 31, 2011·Proceedings of the National Academy of Sciences of the United States of America·Christian P RobertNatesh S Pillai
Nov 5, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Mike HolcombeDavid Worth
Nov 24, 2011·Bulletin of Mathematical Biology·Benjamin J BinderDongcheng Zhang
Jul 6, 2012·Development·Rebecca McLennanPaul M Kulesa
Sep 22, 2012·Biophysical Journal·Daniela K SchlüterMark A J Chaplain
Feb 6, 2013·PLoS Computational Biology·Juliane LiepeMichael P H Stumpf
Feb 22, 2013·Journal of the Royal Society, Interface·Matthew J SimpsonRuth E Baker
Sep 17, 2013·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Benjamin J Binder, Matthew J Simpson
Dec 11, 2013·Statistical Applications in Genetics and Molecular Biology·Dennis PrangleNigel P French
Mar 13, 2014·Journal of Theoretical Biology·D J G AgnewBenjamin J Binder
May 23, 2014·Journal of the Royal Society, Interface·Stuart T JohnstonD L Sean McElwain
Aug 20, 2014·Current Opinion in Cell Biology·Kevin J Cheung, Andrew J Ewald
Aug 19, 2015·Developmental Biology·Rebecca McLennanPaul M Kulesa
Sep 26, 2015·Physical Biology·Phoebe J M JonesJuliane Liepe
Oct 21, 2015·Frontiers in Physiology·Jennifer A FleggD L Sean McElwain
Dec 8, 2015·PLoS Computational Biology·Brenda N VoGraeme J Pettet
Jan 7, 2016·Nature Communications·Richard L MortChristian A Yates
Apr 18, 2016·Journal of Theoretical Biology·Stuart T JohnstonMatthew J Simpson

Citations

Sep 19, 2018·Royal Society Open Science·Andrew ParkerRuth E Baker
Jul 24, 2019·Physical Review. E·George Chappelle, Christian A Yates
Oct 13, 2018·Bulletin of Mathematical Biology·Eduard Campillo-FunolletAnotida Madzvamuse
Apr 9, 2019·Journal of the Royal Society, Interface·David J WarneMatthew J Simpson

Related Concepts

Tissue Adhesions
Cell Communication
Cell Motility
Experimental Design
Size
Simulation
Research Study
Pharmacologic Substance
Computed (Procedure)
Transcriptional Activation Domain

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