Using Centromere Mediated Genome Elimination to Elucidate the Functional Redundancy of Candidate Telomere Binding Proteins in Arabidopsis thaliana

Frontiers in Genetics
Nick Fulcher, Karel Riha

Abstract

Proteins that bind to telomeric DNA form the key structural and functional constituents of telomeres. While telomere binding proteins have been described in the majority of organisms, their identity in plants remains unknown. Several protein families containing a telomere binding motif known as the telobox have been previously described in Arabidopsis thaliana. Nonetheless, functional evidence for their involvement at telomeres has not been obtained, likely due to functional redundancy. Here we performed genetic analysis on the TRF-like family consisting of six proteins (TRB1, TRP1, TRFL1, TRFL2, TRFL4, and TRF9) which have previously shown to bind telomeric DNA in vitro. We used haploid genetics to create multiple knock-out plants deficient for all six proteins of this gene family. These plants did not exhibit changes in telomere length, or phenotypes associated with telomere dysfunction. This data demonstrates that this telobox protein family is not involved in telomere maintenance in Arabidopsis. Phylogenetic analysis in major plant lineages revealed early diversification of telobox proteins families indicating that telomere function may be associated with other telobox proteins.

References

Feb 20, 1997·Nature·B van Steensel, T de Lange
Feb 26, 1998·Cell·B van SteenselT de Lange
Jan 29, 2000·The Plant Journal : for Cell and Molecular Biology·D TremousaygueB Lescure
Apr 3, 2004·Genome Génome / Conseil National De Recherches Canada·Petra SchrumpfováJirí Fajkus
Jul 20, 2004·The Plant Cell·Eugene V Shakirov, Dorothy E Shippen
Sep 15, 2004·The Journal of Biological Chemistry·Zemfira N KaramyshevaDorothy E Shippen
Jun 22, 2005·Nature Cell Biology·Giulia B Celli, Titia de Lange
Sep 17, 2005·Genes & Development·Titia de Lange
Mar 27, 2007·Biochimica Et Biophysica Acta·Barbara Zellinger, Karel Riha
Dec 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·Xiangyu SongDorothy E Shippen
Mar 26, 2010·Nature·Maruthachalam Ravi, Simon W L Chan
Jun 29, 2010·FEBS Letters·J Matthew Watson, Karel Riha
Jun 21, 2011·Journal of Plant Physiology·Ryo MoriguchiYoshinori Kanayama
Mar 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Danelle K SeymourJulin N Maloof
Jul 20, 2012·Genes & Development·Anita KazdaKarel Riha
Jan 9, 2014·The Plant Journal : for Cell and Molecular Biology·Petra Procházková SchrumpfováJiří Fajkus
Nov 2, 2014·Nature Communications·Maruthachalam RaviSimon W L Chan

❮ Previous
Next ❯

Citations

Nov 9, 2017·Frontiers in Plant Science·Guijun YanChunji Liu
Jan 19, 2019·Cells·Petra Procházková SchrumpfováJiří Fajkus
Jul 26, 2016·Frontiers in Plant Science·Petra Procházková SchrumpfováJiří Fajkus
Jun 28, 2018·Plant Molecular Biology·Ladislav DokládalEva Sýkorová

❮ Previous
Next ❯

Methods Mentioned

BETA
genotyping
PCR
electrophoresis
Immunoprecipitation

Software Mentioned

BLAST
ClustalW
TeloTool
CLC Main Workbench

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Plant Journal : for Cell and Molecular Biology
Petra Procházková SchrumpfováJirí Fajkus
The Plant Journal : for Cell and Molecular Biology
Hyun Hee YooIn Kwon Chung
Proceedings of the National Academy of Sciences of the United States of America
M E Gallego, C I White
© 2022 Meta ULC. All rights reserved