Using Colonization Assays and Comparative Genomics To Discover Symbiosis Behaviors and Factors in Vibrio fischeri.

MBio
Clotilde BongrandEdward G Ruby

Abstract

The luminous marine Gram-negative bacterium Vibrio (Aliivibrio) fischeri is the natural light organ symbiont of several squid species, including the Hawaiian bobtail squid, Euprymna scolopes, and the Japanese bobtail squid, Euprymna morsei Work with E. scolopes has shown how the bacteria establish their niche in the light organ of the newly hatched host. Two types of V. fischeri strains have been distinguished based upon their behavior in cocolonization competition assays in juvenile E. scolopes, i.e., (i) niche-sharing or (ii) niche-dominant behavior. This study aimed to determine whether these behaviors are observed with other V. fischeri strains or whether they are specific to those isolated from E. scolopes light organs. Cocolonization competition assays between V. fischeri strains isolated from the congeneric squid E. morsei or from other marine animals revealed the same sharing or dominant behaviors. In addition, whole-genome sequencing of these strains showed that the dominant behavior is polyphyletic and not associated with the presence or absence of a single gene or genes. Comparative genomics of 44 squid light organ isolates from around the globe led to the identification of symbiosis-specific candidates in the genome...Continue Reading

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Citations

Jun 6, 2021·Nature Reviews. Microbiology·Karen L VisickEdward G Ruby
Aug 15, 2021·Nature Reviews. Genetics·Julie Perreau, Nancy A Moran

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Methods Mentioned

BETA
PCR
fluorescence dissecting

Software Mentioned

OrthoMCL
CLC Genomics Workbench
linsi
SEED
trimAl
NS - I
mafft
RAxML
CLC assembler
Prodigal

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