Using enantioselective indicator displacement assays to determine the enantiomeric excess of alpha-amino acids.

Journal of the American Chemical Society
Diana LeungEric V Anslyn

Abstract

Enantioselective indicator displacement assays (eIDAs) were used for the determination of enantiomeric excess (ee) of alpha-amino acids as an alternative to the labor-intensive technique of chromatography. In this study, eIDAs were implemented by the use of two chiral receptors [(Cu(II)(1)](2+), [Cu(II)(2)](2+)) in conjunction with the indicator chrome azurol S. The two receptors were able to enantioselectively discriminate 13 of the 17 analyzed alpha-amino acids. Enantiomeric excess calibration curves were made using both receptors and then used to analyze true test samples to check the system's ability to determine ee accurately. The proposed method uses a conventional UV-vis spectrophotometer to monitor the colorimetric signal, which allows for a potential high-throughput screening (HTS) method for determining ee. The techniques created consistently produced results accurate enough for rapid preliminary determination of ee.

Citations

Jan 8, 2011·Journal of Fluorescence·Yubo WuJianzhang Zhao
May 8, 2010·Journal of the American Chemical Society·Xi ChenLin Pu
Jun 12, 2010·Journal of the American Chemical Society·Giorgio MirriJames H R Tucker
Jul 26, 2011·Journal of the American Chemical Society·Leo A JoyceEric V Anslyn
Aug 1, 2009·Journal of the American Chemical Society·Tianzhi ZhangEric V Anslyn
Mar 5, 2011·The Journal of Organic Chemistry·Takuya MatsumotoTetsuo Nagano
Feb 19, 2009·The Journal of Organic Chemistry·Hai-Ming Guo, Fujie Tanaka
Jun 12, 2010·Chemical Communications : Chem Comm·Matthew J GreaneyLawrence D Margerum
Oct 18, 2012·Journal of Biomolecular Structure & Dynamics·Yan LiuLing Song
Feb 7, 2014·Chemical Reviews·Xin ZhangJuyoung Yoon
Jan 24, 2015·Analytical and Bioanalytical Chemistry·Francesca DiniIngemar Lundström
Dec 15, 2017·Current Protocols in Chemical Biology·Robert A Swyka, David B Berkowitz
Sep 6, 2011·Chemical Society Reviews·Diana LeungEric V Anslyn
Mar 1, 2012·Chemistry : a European Journal·Roy N DsouzaWerner M Nau
Feb 20, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·Huiyeong JuYoichi Habata
May 2, 2019·Scientific Reports·Angel Resendez, Sanjay V Malhotra
Jul 9, 2020·Chemical Communications : Chem Comm·Sara SheykhiPavel Anzenbacher
Jul 30, 2011·Chemical Society Reviews·Ying Zhou, Juyoung Yoon
Apr 19, 2011·Organic & Biomolecular Chemistry·Andrea GualandiJacek Gawroński
May 29, 2013·Chemistry : a European Journal·Dong-Sheng GuoYu Liu
Nov 29, 2014·Angewandte Chemie·Amir NorouzyWerner M Nau

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