Using expanded genetic alphabets to simplify high-throughput genetic testing

Expert Review of Molecular Diagnostics
James R Prudent

Abstract

DNA is the only chemistry that allows for molecular recognition on demand. Unlike any other molecular recognition chemistry, DNA enables the simple design and rapid synthesis of molecule sets that will recognize each other and self-assemble into nanostructures. In molecular diagnostics, DNA is used to capture complementary sequences in order to decode complex mixes. Expanding DNA chemistry to include additional base pairs enables a more precise manipulation of nanostructures constructed with DNA. MultiCode technology is that type of expanded DNA chemistry. The technology exploits DNA hydrogen bonding patterns that differ from natural DNA, thereby enabling a simple means of transcending problems that are otherwise unsolvable. Made up of additional base pairs (not simply single bases), MultiCode technology is used today to decode sequences in an orthogonal manner to natural DNA. This review will discuss MultiCode technology and specifically focus on how the technology can be used to build molecular testing platforms.

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Citations

Jan 26, 2008·Journal of the American Chemical Society·Aaron M LeconteFloyd E Romesberg
Mar 5, 2009·Journal of the American Chemical Society·Young Jun SeoFloyd E Romesberg
Nov 29, 2011·Nucleic Acids Research·Rie YamashigeIchiro Hirao
Jun 29, 2007·Journal of Clinical Microbiology·Frederick S NolteJames R Prudent
Sep 26, 2009·Chembiochem : a European Journal of Chemical Biology·Haige LuEric T Kool
Jun 23, 2009·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Stephen SpellmanThomas M Ellis
Apr 6, 2011·Expert Review of Molecular Diagnostics·Michiko KimotoIchiro Hirao
Nov 22, 2007·Journal of the American Chemical Society·Ichiro HiraoShigeyuki Yokoyama

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