Using focus ion beam to prepare crystal lamella for electron diffraction

Journal of Structural Biology
Heng ZhouXueming Li

Abstract

Electron diffraction provides a powerful tool to solve the structures of small protein crystals. However, strong interactions between the electrons and the materials limit the application of the electron crystallographic method on large protein crystals with micrometer or larger sizes. Here, we used the focused ion beam (FIB) equipped on the scanning electron microscope (SEM) to mill a large crystal to thin lamella. The influences of the milling on the crystal lamella were observed and investigated, including radiation damage on the crystal surface during the FIB imaging, deformation of the thin crystal lamella, and variation in the diffraction intensities under electron radiation. These observations provide important information to optimize the FIB milling, and hence is important to obtain high-quality crystal samples for routine structure determination of protein crystals using the electron cryo-microscope.

Citations

Aug 4, 2020·Acta Crystallographica. Section D, Structural Biology·Logan S RichardsJose A Rodriguez
Aug 29, 2019·Science Advances·Hongyi XuMartin Högbom
Jul 18, 2020·F1000Research·Michael C ThompsonJose A Rodriguez
Aug 28, 2020·Frontiers in Molecular Biosciences·Emma V BealePeijun Zhang
Dec 10, 2020·Proceedings of the National Academy of Sciences of the United States of America·Michael W MartynowyczTamir Gonen
Feb 5, 2021·Emerging Topics in Life Sciences·Jason E SchafferJoseph M Jez
Mar 2, 2021·Acta Crystallographica. Section D, Structural Biology·Max T B Clabbers, Hongyi Xu
Jun 22, 2021·Annual Review of Biochemistry·Xuelang MuTamir Gonen
Aug 21, 2021·Nature Communications·Jingjing ZhaoXiaodong Zou
Dec 8, 2021·Proceedings of the National Academy of Sciences of the United States of America·Michael W MartynowyczTamir Gonen

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