Apr 20, 2020

A Fast High-Affinity Fluorescent Serotonin Biosensor Engineered from a Tick Lipocalin

BioRxiv : the Preprint Server for Biology
S. ZhangHuiwang Ai

Abstract

Serotonin (5-HT) is an important signaling monoamine in and beyond the central nervous system (CNS). We report structure-guided engineering of a green fluorescent, genetically encoded serotonin sensor (G-GESS) from a 5-HT-binding lipocalin in the soft tick. G-GESS shows fast response kinetics and high affinity, specificity, brightness, and photostability. We used G-GESS to image 5-HT dynamics in vitro and in behaving mice, demonstrating the broad utility of this novel biosensor.

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