Using HTK for prolonged pancreas preservation prior to human islet isolation

The Journal of Surgical Research
José Caballero-CorbalánDaniel Brandhorst

Abstract

Histidine-tryptophan-ketoglutarate (HTK) has been established as an alternative to University-of-Wisconsin solution (UWS) for abdominal organ preservation, but data about HTK efficiency to preserve pancreata during prolonged cold ischemia time (CIT) are conflicting. In human islet transplantation, HTK provided similar isolation outcomes after short CIT. The present study aimed to investigate whether islets can be successfully isolated from HTK-preserved pancreata after prolonged CIT compared with UWS. Sixty-four human pancreata retrieved from donors meeting criteria for kidney donation were perfused utilizing either HTK or UWS and preserved for more or less than 10 h prior to islet isolation. Along with parameters related to isolation and islet quality assessment, the dry-to-wet weight ratio was evaluated. Donor- and procurement-related factors did not vary between HTK- and UWS-perfused pancreata. The dry-to-wet weight ratio was lower in HTK-preserved pancreata indicated tissue edema (21.0% ± 3.5% versus 24.8% ± 2.0%, P = 0.007). Isolation-related variables differed between experimental groups after prolonged CIT with respect to purified packed tissue volume (9.1 ± 5.0 versus 17.2 ± 8.1 μL/g, P = 0.004) and islet yield (1910 ± ...Continue Reading

References

Jan 1, 1979·Journal of Wildlife Diseases·W J Adrian, M L Stevens
Jul 1, 1990·Acta diabetologica latina·C RicordiR Alejandro
Oct 1, 1980·The Thoracic and Cardiovascular Surgeon·H J Bretschneider
Aug 31, 2002·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Stefan G TulliusPeter Neuhaus
Oct 13, 2004·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Nils A 't HartRutger J Ploeg
Feb 1, 2006·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Richard S MangusJonathan A Fridell
Apr 26, 2006·Clinical and Experimental Immunology·T LinnD Brandhorst
Oct 13, 2006·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Gero PuhlPeter Neuhaus
Feb 14, 2007·Transplantation·Gerhard Opelz, Bernd Döhler
Sep 13, 2008·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·D AlonsoR B Stevens
Oct 29, 2008·Transplant International : Official Journal of the European Society for Organ Transplantation·Stefan SchneebergerAlfred Königsrainer
Dec 11, 2008·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Z A StewartD L Segev
Feb 10, 2009·Transplantation·Heide BrandhorstDaniel Brandhorst
Mar 21, 2009·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Z A StewartD L Segev
Jan 1, 2008·Cell Transplantation·A S FribergD Brandhorst
Apr 1, 2010·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J A FridellJ A Powelson

❮ Previous
Next ❯

Citations

Mar 29, 2013·Apoptosis : an International Journal on Programmed Cell Death·Daniel BrandhorstPaul R V Johnson
Mar 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Barbara LudwigUriel Barkai
Dec 19, 2019·Diabetes/metabolism Research and Reviews·Laura NigiGuido Sebastiani
Dec 30, 2014·American Journal of Physiology. Endocrinology and Metabolism·Meirigeng QiIsmail H Al-Abdullah
Feb 11, 2021·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Jason B DoppenbergEelco J P de Koning
May 27, 2021·Transplant International : Official Journal of the European Society for Organ Transplantation·Jason B DoppenbergHenri G D Leuvenink

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.