Using In Vitro Immunomodulatory Properties of Lactic Acid Bacteria for Selection of Probiotics against Salmonella Infection in Broiler Chicks

PloS One
Junchang FengXin Zhao

Abstract

Poultry is known to be a major reservoir of Salmonella. The use of lactic acid bacteria has become one of successful strategies to control Salmonella in poultry. The purpose of this study was to select lactic acid bacteria strains by their in vitro immunomodulatory properties for potential use as probiotics against Salmonella infection in broiler chicks. Among 101 isolated lactic acid bacteria strains, 13 strains effectively survived under acidic (pH 2.5) and bile salt (ranging from 0.1% to 1.0%) conditions, effectively inhibited growth of 6 pathogens, and adhered to Caco-2 cells. However, their in vitro immunomodulatory activities differed significantly. Finally, three strains with higher in vitro immunomodulatory properties (Lactobacillus plantarum PZ01, Lactobacillus salivarius JM32 and Pediococcus acidilactici JH231) and three strains with lower in vitro immunomodulatory activities (Enterococcus faecium JS11, Lactobacillus salivarius JK22 and Lactobacillus salivarius JM2A1) were compared for their inhibitory effects on Salmonella adhesion and invasion to Caco-2 cells in vitro and their antimicrobial effects in vivo. The former three strains inhibited Salmonella adhesion and invasion to Caco-2 cells in vitro, reduced the num...Continue Reading

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Citations

Sep 8, 2019·Nature Communications·Haibo MuJinyou Duan
Jul 18, 2017·Probiotics and Antimicrobial Proteins·Mattika AbhisinghaChetsadaporn Pitaksutheepong
Apr 4, 2021·Animals : an Open Access Journal From MDPI·Yordan MartínezManuel Valdivié
May 16, 2021·The Journal of Microbiology·Zhenzhen LiShancen Zhao
Jun 3, 2021·Poultry Science·Rim El JeniTodd R Callaway
Aug 14, 2021·Veterinary Microbiology·Vladimir I TrukhachevPeter V Zolotukhin

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Datasets Mentioned

BETA
AY765397
DQ393267
DQ202328

Methods Mentioned

BETA
PCR
enzyme-linked immunosorbent assay
ELISA

Software Mentioned

Basic Local Alignment Search Tool ( BLAST )
SPSS

Related Concepts

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