Using laser tweezers to measure twitching motility in Neisseria

Current Opinion in Microbiology
Berenike Maier


Dynamic properties of type IV pili are essential for their function in bacterial infection, twitching motility and gene transfer. Laser tweezers are versatile tools to study the molecular mechanism underlying pilus dynamics at the single molecule level. Recently, these optical tweezers have been used to monitor pilus elongation and retraction in vivo at a resolution of several nanometers. The force generated by type IV pili exceeds 100 pN making pili the strongest linear motors characterized to date. The study of pilus dynamics at the single molecule level sheds light on kinetics, force generation, switching and mechanics of the Neisseria gonorrhoeae pilus motor.


Sep 1, 1972·Journal of General Microbiology·D E Bradley
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·R M Berry, H C Berg
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M WolfgangMichael Koomey
Jul 13, 2000·The Journal of Cell Biology·R D Vale
Nov 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·M N LiangG M Whitesides
Nov 21, 2000·Current Biology : CB·D Kaiser
Jun 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·J M Skerker, H C Berg
Mar 20, 2002·The Journal of Cell Biology·Shaun W LeeMagdalene So
Nov 12, 2002·Journal of Bacteriology·Timothy J HerdendorfKatrina T Forest
Jun 27, 2003·Nature·Karen A FahrnerH C Berg
Aug 15, 2003·Nature·David G Grier
Sep 19, 2003·Current Biology : CB·Alex Mogilner, George Oster
Mar 31, 2004·Molecular Microbiology·Lynette J CrowtherMichael S Donnenberg
Apr 22, 2004·Nature Reviews. Microbiology·Lisa CraigJohn A Tainer
Apr 23, 2004·Acta Crystallographica. Section D, Biological Crystallography·Katrina T ForestTimothy J Herdendorf
Apr 23, 2004·The EMBO Journal·Philippe MorandXavier Nassif
Jun 2, 2004·Journal of Molecular Microbiology and Biotechnology·Eric Nudleman, D Kaiser
Jun 9, 2004·Nature Structural & Molecular Biology·Berenike MaierMichael P Sheetz
Jul 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Berenike MaierMichael P Sheetz
Sep 21, 2004·Biophysical Journal·Jana JassOve Axner
Aug 1, 2006·The Review of Scientific Instruments·Keir C Neuman, Steven M Block


Apr 3, 2008·Journal of the Royal Society, Interface·Hu Zhang, Kuo-Kang Liu
Jun 15, 2007·Infection and Immunity·Johanna K HansenKatrina T Forest
May 12, 2009·Journal of Bacteriology·Martin ClausenBerenike Maier
Mar 4, 2008·Journal of Bacteriology·Xiaoyan HanJulian I Rood
Oct 28, 2009·Journal of Molecular Biology·L M SampaleanuP L Howell
Feb 3, 2009·Biophysical Journal·Martin ClausenBerenike Maier
Feb 27, 2009·FEMS Microbiology Reviews·Jean-Francois Allemand, Berenike Maier
Jun 2, 2009·Vaccine·Etienne CarbonnelleMumtaz Virji
Apr 15, 2008·Biochimica Et Biophysica Acta·Bart Hazes, Laura Frost
Aug 21, 2014·Frontiers in Microbiology·Alexandra K PerrasChristine Moissl-Eichinger
May 19, 2012·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Marcelo B AntunesNoam A Cohen
Mar 7, 2009·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Dirk OpitzBerenike Maier

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Separation Anxiety

Separation anxiety is a type of anxiety disorder that involves excessive distress and anxiety with separation. This may include separation from places or people to which they have a strong emotional connection with. It often affects children more than adults. Here is the latest research on separation anxiety.

KIF1A Associated Neurological Disorder

KIF1A associated neurological disorder (KAND) is a rare neurodegenerative condition caused by mutations in the KIF1A gene. KAND may present with a wide range and severity of symptoms including stiff or weak leg muscles, low muscle tone, a lack of muscle coordination and balance, and intellectual disability. Find the latest research on KAND here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Berenike MaierMichael P Sheetz
Proceedings of the National Academy of Sciences of the United States of America
Berenike MaierMichael P Sheetz
Proceedings of the National Academy of Sciences of the United States of America
J M Skerker, H C Berg
© 2021 Meta ULC. All rights reserved