Using lectins to identify hidden antigens in Fasciola hepatica

Journal of Helminthology
H C McAllisterD P Knox

Abstract

Fasciola hepatica is the causative agent of fascioliasis, one of the most economically important helminth diseases of livestock worldwide. Traditionally, fascioliasis has been controlled by the strategic use of fasciolicidal drugs, but the emergence of resistant parasites has spurred an interest in developing vaccines as an alternative means of control. Most vaccine studies to date have evaluated conventional antigens, which are exposed to the host's immune system during the course of a natural infection. 'Hidden' antigens have proven to be effective vaccine candidates in other parasite species, most notably the blood-feeding nematode parasite, Haemonchus contortus, and tend to be expressed in the intestine or gut of the parasite. Fasciola hepatica is known to ingest large quantities of blood and may be vulnerable to this approach. Most, if not all, of the candidate antigens identified thus far have been membrane-bound glycoproteins which were solubilized by detergents. Here, we have attempted to employ lectins to select gut-associated glycoproteins from complex mixtures of somatic extracts of adult F. hepatica. We have conducted a comprehensive lectin-binding screen on adult histological sections with a panel of 16 fluorescent...Continue Reading

References

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Mar 1, 2010·Animal : an International Journal of Animal Bioscience·J van DijkP J Skuce

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Citations

Apr 12, 2016·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Eman H Abdel-RahmanEman E El Shanawany
Jul 29, 2016·Molecular & Cellular Proteomics : MCP·Alessandra RavidàSandra M O'Neill
Jan 1, 2016·PLoS Neglected Tropical Diseases·Ernesto RodríguezTeresa Freire

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