Using linkage-specific monoclonal antibodies to analyze cellular ubiquitylation

Methods in Molecular Biology
Kim NewtonVishva M Dixit

Abstract

Antibodies that specifically recognize polyubiquitin chains containing ubiquitins linked at a particular lysine residue are powerful tools for interrogating endogenous protein modifications. Here, we describe protocols for revealing K11-, K48-, and K63-linked polyubiquitin chains by western blotting, immunoprecipitation, or immunostaining.

Citations

Jan 16, 2013·The Journal of Cell Biology·Michael LazarouRichard J Youle
Feb 16, 2016·Structure·Simin RahighiSoichi Wakatsuki
Nov 28, 2012·Nature Chemical Biology·Yingnan ZhangJacob E Corn
Jan 23, 2019·EMBO Reports·Sjoerd Jl van WijkMike Heilemann
Oct 23, 2013·Journal of Cancer Research and Clinical Oncology·Manuel DebaldGisela Walgenbach-Brünagel
Mar 11, 2020·Frontiers in Chemistry·Ila van KruijsbergenFred van Leeuwen
Mar 23, 2018·Nature Communications·David S HewingsIngrid E Wertz
Mar 17, 2020·Frontiers in Molecular Biosciences·Katarzyna Kliza, Koraljka Husnjak

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