Using low-risk factors to generate non-integrated human induced pluripotent stem cells from urine-derived cells

Stem Cell Research & Therapy
Linli WangJun Li

Abstract

Because the lack of an induced pluripotent stem cell (iPSC) induction system with optimal safety and efficiency limits the application of these cells, development of such a system is important. To create such an induction system, we screened a variety of reprogrammed plasmid combinations and multiple compounds and then verified the system's feasibility using urine cells from different individuals. We also compared large-scale iPSC chromosomal variations and expression of genes associated with genomic stability between this system and the traditional episomal system using karyotype and quantitative reverse transcription polymerase chain reaction analyses. We developed a high-efficiency episomal system, the 6F/BM1-4C system, lacking tumorigenic factors for human urine-derived cell (hUC) reprogramming. This system includes six low-risk factors (6F), Oct4, Glis1, Klf4, Sox2, L-Myc, and the miR-302 cluster. Transfected hUCs were treated with four compounds (4C), inhibitor of lysine-demethylase1, methyl ethyl ketone, glycogen synthase kinase 3 beta, and histone deacetylase, within a short time period. Comparative analysis revealed significantly decreased chromosomal variation in iPSCs and significantly increased Sirt1 expression comp...Continue Reading

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Citations

Nov 15, 2019·Cell Transplantation·Aline Yen Ling Wang, Charles Yuen Yung Loh
May 8, 2020·Cardiology Research and Practice·Ping HuangPing Zhu
Jun 5, 2018·Intractable & Rare Diseases Research·Kaixuan XingJinxiang Han
Dec 20, 2018·Journal of Cellular Physiology·Bharath Kumar VelmuruganRathinasamy Baskaran
Sep 22, 2020·Current Protocols in Stem Cell Biology·Marga J BoumaChristian Freund
May 15, 2021·Cell & Bioscience·Tianyuan Shi, Martin Cheung

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Datasets Mentioned

BETA
GSE85885

Methods Mentioned

BETA
transfection
transfections
PCR
flow cytometry
reverse transcription PCR
electrophoresis

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