Using metabarcoding to compare the suitability of two blood-feeding leech species for sampling mammalian diversity in North Borneo

Molecular Ecology Resources
Rosie DrinkwaterStephen J Rossiter

Abstract

The application of high-throughput sequencing (HTS) for metabarcoding of mixed samples offers new opportunities in conservation biology. Recently, the successful detection of prey DNA from the guts of leeches has raised the possibility that these, and other blood-feeding invertebrates, might serve as useful samplers of mammals. Yet little is known about whether sympatric leech species differ in their feeding preferences, and whether this has a bearing on their relative suitability for monitoring local mammalian diversity. To address these questions, we collected spatially matched samples of two congeneric leech species Haemadipsa picta and Haemadipsa sumatrana from lowland rainforest in Borneo. For each species, we pooled ~500 leeches into batches of 10 individuals, performed PCR to target a section of the mammalian 16S rRNA locus and undertook sequencing of amplicon libraries using an Illumina MiSeq. In total, we identified sequences from 14 mammalian genera, spanning nine families and five orders. We found greater numbers of detections, and higher diversity of OTUs, in H. picta compared with H. sumatrana, with rodents only present in the former leech species. However, comparison of samples from across the landscape revealed n...Continue Reading

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Citations

Nov 15, 2018·Molecular Ecology·Elizabeth L ClareM Brock Fenton
Jul 2, 2020·Ecology and Evolution·Kaori MorishimaMineaki Aizawa
Nov 26, 2020·Proceedings of the National Academy of Sciences of the United States of America·Nicolas S LocatelliDiana S Baetscher
Apr 15, 2021·PeerJ·Elizabeth L ClareJoanne E Littlefair
Aug 18, 2021·Molecular Ecology Resources·Christina LynggaardKristine Bohmann
Sep 23, 2021·Molecular Ecology Resources·Kristine BohmannSimon Creer

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Methods Mentioned

BETA
PCR
Assay
PCRs

Software Mentioned

sumaclust
aliview
adapterremoval
vegan
PERMANOVA
LULU
inext
NMDS
R
picta

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