Using metabolomics to assess the sub-lethal effects of zinc and boscalid on an estuarine polychaete worm over time

Metabolomics : Official Journal of the Metabolomic Society
Georgia M SinclairSara M Long

Abstract

Zinc is a heavy metal commonly detected in urban estuaries around Australia. Boscalid is a fungicide found in estuaries, both in water and sediment, it enters the system predominantly through agricultural run-off. Zinc is persistent while boscalid breaks down, with a half-life of 108 days. Both contaminants are widely distributed and their effects on ecosystems are not well understood. The aim of this study was to determine the metabolite changes in Simplisetia aequisetis (an estuarine polychaete) following laboratory exposure to a sub-lethal concentration of zinc or boscalid over a 2-week period. Individuals were collected at six time points over a 2-week period. Whole polychaete metabolites were extracted and quantified using a multi-platform approach. Polar metabolites were detected using a semi-targeted GC-MS analysis and amine containing compounds were analysed using a targeted LC-MS analysis. Total lipid energy content was also analysed for Simplisetia aequisetis. The pathways that responded to zinc and boscalid exposure were alanine, aspartate and glutamate metabolism (AAG); glycine, serine and threonine metabolism (GST) and metabolites associated with the tricarboxylic acid cycle (TCA). Results showed that changes in to...Continue Reading

References

Mar 26, 2003·Ecotoxicology and Environmental Safety·Ross V Hyne, William A Maher
May 14, 2005·Antioxidants & Redox Signaling·Yasoo SugiuraMakoto Suematsu
Nov 1, 2005·Drug Discovery Today·Wolfram Weckwerth, Katja Morgenthal
Jun 10, 2006·Aquatic Toxicology·Linda M SamuelssonD G Joakim Larsson
Sep 16, 2006·Ecology Letters·Rick Relyea, Jason Hoverman
Jan 13, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Adam HinesMark R Viant
Jan 15, 2010·Environmental Pollution·Stephen MarshallAry Hoffmann
Sep 2, 2011·Analytical Chemistry·Berin A BoughtonUte Roessner
Oct 22, 2011·Environmental Pollution·Joan ArtigasRichard Williams
Sep 1, 2007·Metabolomics : Official Journal of the Metabolomic Society·Lloyd W SumnerMark R Viant
Oct 3, 2014·Genome Biology and Evolution·João Henrique Frota CavalcantiWagner L Araújo
Nov 13, 2014·Methods in Molecular Biology·Eleanor C SaundersMalcolm J McConville
Sep 29, 2015·Environmental Pollution·Andreia C M RodriguesJoão L T Pestana
Nov 28, 2015·Metabolomics : Official Journal of the Metabolomic Society·Anne Julie OvergaardFlemming Pociot
Dec 5, 2015·Journal of Cell Communication and Signaling·Soujanya D YelamanchiHarsha Gowda
Apr 26, 2016·The Science of the Total Environment·Cristian Gómez-CanelaLeon P Barron
Jul 12, 2016·Environmental Pollution·David J SharleyVincent J Pettigrove
Oct 30, 2016·Regulatory Toxicology and Pharmacology : RTP·B van RavenzwaayT Walk
Aug 22, 2017·Journal of Pharmaceutical and Biomedical Analysis·Danuta DudzikCoral Barbas

❮ Previous
Next ❯

Citations

Feb 27, 2020·Metabolomics : Official Journal of the Metabolomic Society·Satabdi GangulyBimal P Mohanty
Aug 8, 2020·Water Environment Research : a Research Publication of the Water Environment Federation·Chunying LiChein-Chi Chang

❮ Previous
Next ❯

Software Mentioned

R
MetaboAnalyst
Agilent MassHunter Quantitative Analysis
PERMANOVA
Shimadzu Lab Solutions

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.