Using msa-2b as a molecular marker for genotyping Mexican isolates of Babesia bovis

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
Alma D GenisJ V Figueroa

Abstract

Variable merozoite surface antigens of Babesia bovis are exposed glycoproteins having a role in erythrocyte invasion. Members of this gene family include msa-1 and msa-2 (msa-2c, msa-2a(1), msa-2a(2) and msa-2b). To determine the sequence variation among B. bovis Mexican isolates using msa-2b as a genetic marker, PCR amplicons corresponding to msa-2b were cloned and plasmids carrying the corresponding inserts were purified and sequenced. Comparative analysis of nucleotide and deduced amino acid sequences revealed distinct degrees of variability and identity among the coding gene sequences obtained from 16 geographically different Mexican B. bovis isolates and a reference strain. Clustal-W multiple alignments of the MSA-2b deduced amino acid sequences performed with the 17 B. bovis Mexican isolates, revealed the identification of three genotypes with a distinct set each of amino acid residues present at the variable region: Genotype I represented by the MO7 strain (in vitro culture-derived from the Mexico isolate) as well as RAD, Chiapas-1, Tabasco and Veracruz-3 isolates; Genotype II, represented by the Jalisco, Mexico and Veracruz-2 isolates; and Genotype III comprising the sequences from most of the isolates studied, Tamaulip...Continue Reading

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Citations

Jun 12, 2010·Transboundary and Emerging Diseases·J PerezJ V Figueroa
Jan 13, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Naoaki YokoyamaDinh Thi Bich Lan
Nov 2, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Nilukshi LiyanagunawardenaNaoaki Yokoyama
Sep 18, 2013·Parasitology International·Muncharee TattiyapongNaoaki Yokoyama
Jun 11, 2016·Veterinary World·Biswa Ranjan MaharanaNaduvanahalli Rajanna Sudhakar
Dec 7, 2017·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Carlos António MatosRosangela Zacarias Machado
Jul 1, 2015·Nature Cell Biology·Sascha Martens, Andreas Bachmair
May 13, 2019·Parasitology Research·Natalia Serra MendesMarcos Rogério André
Nov 26, 2020·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Carlos António MatosRosangela Zacarias Machado

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