Using near-isogenic barley lines to validate deoxynivalenol (DON) QTL previously identified through association analysis

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Stephanie Navara, Kevin P Smith

Abstract

Fusarium head blight (FHB) and its associated mycotoxin, deoxynivalenol (DON), are the major biotic factors limiting cereal production in many parts of the world. A recent association mapping (AM) study of US six-row spring barley identified several modest effect quantitative trait loci (QTL) for DON and FHB. To date, few studies have attempted to verify the results of association analyses, particularly for complex traits such as DON and FHB resistance in barley. While AM methods use measures to control for the effects of population structure and multiple testing, false positive associations may still occur. A previous AM study used elite breeding germplasm to identify QTL for FHB and DON. To verify the results of that study, we evaluated the effects of the nine DON QTL using near-isogenic lines (NILs). We created families of contrasting homozygous haplotypes from lines in the original AM populations that were heterozygous for the DON QTL. Seventeen NIL families were evaluated for FHB and DON in three field experiments. Significant differences between contrasting NIL haplotypes were detected for three QTL across environments and/or genetic backgrounds, thereby confirming QTL from the original AM study. Several explanations for ...Continue Reading

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Citations

Oct 7, 2016·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Pedro Correa BraunerThomas Miedaner
Jun 13, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Wenliang LiZhangying Xi
Nov 8, 2017·Nature Reviews. Genetics·Rebecca NelsonPeter Balint-Kurti
Oct 28, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Karolina Maria Słomińska-DurdasiakJochen Christoph Reif

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