Using Nesprin Tension Sensors to Measure Force on the LINC Complex

Methods in Molecular Biology
Paul T Arsenovic, Daniel E Conway

Abstract

Mechanotransduction, or the process by which mechanical forces regulate cellular functions, is increasingly studied in a variety of different physiological and pathological contexts. Although these forces are most often studied at cell-matrix and cell-cell adhesions, recent work has shown that the nuclear LINC complex is also subject to mechanical forces. Here we describe how to use a FRET-based biosensor, known as TSmod, in the LINC complex protein nesprin-2G. This approach allows for measurement of LINC complex forces in living cells with spatial-temporal resolution.

Citations

Nov 30, 2019·Nature Reviews. Cancer·Gautier FollainJacky G Goetz
Nov 14, 2019·Cellular and Molecular Bioengineering·Carl R MayerDaniel E Conway
Apr 21, 2021·Nature Biomedical Engineering·Cierra J WalkerKristi S Anseth

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