Using non-thermal irreversible electroporation to create an in vivo niche for exogenous cell engraftment

BioTechniques
Tammy T ChangBoris Rubinsky

Abstract

The critical shortage of donor organs has spurred investigation of alternative approaches to either generate replacement organs or implant exogenous cells for treatment of end-stage organ failure. Non-thermal irreversible electroporation (NTIRE), which uses brief high electric field pulses to induce irreversible permeabilization of cell membranes, has emerged as a technique for tumor ablation. Here, we introduce a new application for NTIRE that employs in situ cell ablation to create a niche within a solid organ for engraftment of exogenous cells in vivo. We treated the livers of mice with NTIRE and subsequently implanted exogenous congenic hepatocytes within the zone of cell ablation. Donor hepatocytes engrafted and integrated with host liver parenchyma pre-treated with NTIRE. This new approach should have value for studying the effects of a native matrix scaffold on in vivo cell growth and may pioneer a new type of minimally-invasive regenerative surgery.

References

Mar 18, 2005·Annals of Biomedical Engineering·R V DavalosB Rubinsky
Jan 24, 2007·Technology in Cancer Research & Treatment·Boris RubinskyPaul Mikus
Aug 3, 2007·Technology in Cancer Research & Treatment·Elad MaorBoris Rubinsky
Sep 8, 2010·Journal of Biomechanical Engineering·Mary PhillipsBoris Rubinsky
Jan 10, 2012·British Journal of Cancer·M A PhillipsB Rubinsky
May 30, 2012·Journal of Hepatology·Keisuke KakisakaGregory J Gores
Jan 18, 2014·Seminars in Interventional Radiology·Govindarajan NarayananKatuska Barbery
Sep 2, 2015·Technology in Cancer Research & Treatment·Liel RubinskyBoris Rubinsky
Jan 13, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Seema S DesaiTammy T Chang
Jan 29, 2016·PeerJ·Alexander GolbergBasak E Uygun
May 16, 2017·The Journal of Surgical Research·Vivian X ZhouTammy T Chang

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Citations

Oct 9, 2018·Biotechnology Progress·Rajesh Guruswamy Damodaran, Patrick Vermette
Dec 27, 2018·Journal of Clinical Medicine·Aman SainiRahmi Oklu
Aug 14, 2020·Cancers·Aleksander KiełbikJulita Kulbacka
Sep 8, 2019·Progress in Retinal and Eye Research·Andri K RiauJodhbir S Mehta
Mar 19, 2019·HPB : the Official Journal of the International Hepato Pancreato Biliary Association·Chenang LyuTammy T Chang

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Software Mentioned

NTIRE

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